河北省唐山市奶犊牛病毒性腹泻流行病学调查

doi:10.16431/j.cnki.1671-7236.2021.11.031

Abstract

Abstract: The purpose of the experiment was to master the epidemic status of viral diarrhea pathogens of dairy calves in 13 counties (cities and districts) of Tangshan city, Hebei province, and to effectively prevent and control dairy calves diarrhea. 788 fecal samples of diarrhea calves from 38 dairy farms in different areas of Tangshan were collected to extract genomic RNA from diarrhea fecal samples. According to the E2 gene of Bovine viral diarrhea virus (BVDV), VP6 gene of Bovine rotavirus (BRV) and N gene of Bovine coronavirus (BCV) published in GenBank, specific primers were designed by Primer Premier 5.0 software, and the three genes in fecal samples were detected by PCR. The pathogen detection results of different areas, seasons and mixed infections were summarized and analyzed by Excel 2007. The positive rates of BVDV, BRV and BCV were 29.82% (235/788), 29.44% (232/788) and 18.02% (142/788), respectively. In all tested areas, Luannan county had the highest BVDV infection rate, with a positive detection rate of 45.38% (59/130). Hangu district had the highest BRV and BCV infection rates, with a positive detection rate of 55.00% (22/40) and 37.50% (15/40), respectively. From the sampling season, the BRV infection rate was the highest in spring, summer and autumn, with the positive detection rates of 27.71% (46/166), 39.58% (114/288) and 19.89% (39/196), respectively. In winter, BVDV infection was the main infection, with the positive detection rate of 52.17% (72/138). In terms of mixed infection, BRV+BCV double mixed infection was the main, and the infection rate was 8.37% (66/788). BVDV, BRV and BCV infection were found in diarrhea calves in Tangshan city, Hebei province, and the main infection was BVDV and BRV.

Key words: dairy calf; viral diarrhea; Bovine viral diarrhea virus (BVDV); Bovine rotavirus (BRV); Bovine coronavirus (BCV); epidemiological investigation

CLC Number:

S852.65⁺3

CHANG Liyun, LIU Zhiyong, QIN Jianhua, ZHAO Yuelan. Epidemiological Investigation of Viral Diarrhea of Dairy Calves in Tangshan City, Hebei Province[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4213-4219.

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Epidemiological Investigation of Viral Diarrhea of Dairy Calves in Tangshan City, Hebei Province

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