新疆喀什地区安格斯犊牛腹泻主要病毒性病原调查及牛诺如病毒全基因序列分析

doi:10.16431/j.cnki.1671-7236.2022.11.033

Abstract

Abstract: 【Objective】 The aim of this experiment was to investigate the prevalence of the main viral pathogens of Angus calves diarrhea in Kashgar, Xinjiang and the genome-wide genetic relationship of Bovine norovirus (BNoV).【Method】 RT-PCR was used to detect four viral pathogens of Bovine rotavirus (BRV), Bovine coronavirus (BCoV), Bovine viral diarrhoea virus (BVDV) and BNoV in 363 calf diarrhea fecal samples collected from 4 cattle farms in Kashgar in different seasons in 2021, and the amplified BNoV gene sequences were analyzed.【Result】 The positive rates of BCoV and BNoV were 36.09% (131/363) and 25.07% (91/363), respectively.In mixed infection, BCoV+BNoV was the most, with a positive rate of 44.00% (22/50).Four fields in Kashgar area were tested.BCoV and BNoV were mainly detected in field A, with a detection rate of 43.52% (47/108) and 32.41% (35/108), respectively.BCoV was mainly detected in field B, with a detection rate of 52.21% (71/136).The pathogen detection rates in fields C and D were generally low.The viruses detected the most in autumn and winter were BNoV and BCoV, with detection rates of 66.67% (50/75) and 77.59% (90/116), respectively.The analysis of the whole gene sequence of BNoV showed that BNoV strain Bo/XJ-KS/01/CHN (accession No.:ON076888) detected in this experiment belonged to BNoV GⅢ.2, and was in the same branch of the evolutionary tree as CN/HB-SJZ-2 and CH/HB/BD/2019 strains in China, and had the highest nucleotide similarities with CN/HB-SJZ-2 strains, which was 93.70%.The nucleotide similarity with Jena/1999/UK strain isolated from Britain were the lowest, which was 72.49%. Further comparison and analysis of three open reading frames (ORFs) showed that the nucleotide and amino acid similarities between the whole genome sequence of BNoV and the domestic reference strain CN/HB-SJZ-2 were mainly the highest in ORF1 region, with 94.24% and 98.69%, respectively.The nucleotide and amino acid similarities with the foreign reference strain Jena/1999/UK was the lowest in ORF3 region, which was 63.59% and 64.57%, respectively.The comparison with domestic and foreign strains showed that there was no gene recombination.【Conclusion】 The epidemic situation of calf diarrhea in Southern Xinjiang was different due to different seasons and fields.The infection rate of viral pathogens was mainly high in autumn and winter, and single BCoV, BRV and BNoV were the main infections.The whole gene sequence of BNoV type GⅢ.2 Bo/XJ-KS/01/CHN determined in this study had the closest relationship with the reference strain CN/HB-SJZ-2 in China, and had no gene recombination phenomenon compared with the whole genome reference sequence at home and abroad.

Key words: calf diarrhea; viral pathogens; epidemiological investigation; genetic evolutionary analysis

CLC Number:

S852.65^＋3

WU Jing, SHI Jincheng, SANIYE Kerban, NAMAN Abudula, SUN Lei, YAO Gang, ZHONG Qi, LUO Liangtao, ZHAO Hongqiong, MA Xuelian. Investigation of the Main Viral Pathogens of Angus Calf Diarrhea in Kashgar Region of Xinjiang and Whole Gene Sequence Analysis of Bovine Norovirus[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4420-4428.

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Investigation of the Main Viral Pathogens of Angus Calf Diarrhea in Kashgar Region of Xinjiang and Whole Gene Sequence Analysis of Bovine Norovirus

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