2013~2018年江西省猪伪狂犬病病毒流行株gE和gB基因遗传变异分析

doi:10.16431/j.cnki.1671-7236.2020.01.020

Abstract

Abstract: In order to grasp the molecular epidemiology and genetic variation of pseudorabies virus (PRV) in Jiangxi province,this study used the established PCR method in the laboratory to amplify gE and gB genes of the collected PRV positive tissue samples from Nanchang,Yichun,Ganzhou,Ji'an,Jiujiang,Shangrao,Fuzhou and Xinyu in Jiangxi province during 2013 to 2018,and the PCR products were sequenced and analyzed.The results showed that a total of 64 PRV gE gene sequences and 12 PRV gB gene sequences were obtained.The phylogenetic tree of gE gene showed that 64 strains of PRV belonged to one large branch,and they were closely related to Asian strains and domestic isolates in recent years,all of which were GⅠ type,but had far-reaching relationship with GⅡ type European and American classic strains;The nucleotide and amino acid homology bewteen Jiangxi strain gE gene and 19 reference strains were 97.2% to 100% and 94.6% to 100%,respectively;The nucleotide and amino acid homologies between gB gene and 11 reference strains were 98.2% to 100% and 96.5% to 100%,respectively;There were base deletions,insertion and site mutations exist in gB gene of Jiangxi strain compared with Bartha-K61 vaccine strain.This study confirmed the prevalence and variation of PRV in Jiangxi province from the perspective of molecular epidemiology,and provided theoretical basis for scientific prevention and control of PRV in Jiangxi province.

Key words: porcine pseudorabies virus (PRV); molecular epidemiological investigation; gE gene; gB gene; variation

CLC Number:

S852.65⁺9.1

GAO Yingxue, JIANG Xinhua, ZHOU Hetian, LUO Feng, YANG Danfeng, WAN Wenzhong, DENG Shunzhou. Genetic Variation Analysis of gE and gB Genes of Pseudorabies Virus in Jiangxi Province During 2013 to 2018[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 164-173.

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Genetic Variation Analysis of gE and gB Genes of Pseudorabies Virus in Jiangxi Province During 2013 to 2018

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