2016－2021年广西H9亚型禽流感病毒HA和NA基因遗传进化动态分析

doi:10.16431/j.cnki.1671-7236.2023.05.022

Abstract

Abstract: 【Objective】 This study was aimed to further understand the evolutionary dynamics and epidemic characteristics of H9 subtype Avian influenza virus (AIV) in Guangxi,which could provide data support for disease prevention strategy constituted and modified.【Method】 3 600 swabs samples of poultry throat,cloaca and environment were collected in several area markets of Guangxi from 2016 to 2021,then were inoculated in 9-11 days age SPF chicken embryos to isolate virus,the hemagglutinin (HA) and neuraminidase (NA) genes of H9 subtype AIV were amplified,sequenced and genetic evolutionary drynamic analyzed based on hemagglutinin and hemagglutinin-inhibit test,and Real-time quantitative RT-PCR detection assays.【Result】 46 HA and NA genes sequence were acquired,the length of HA gene was 1 683 bp,coding for 560 amino acids and 4 NA gene was 1 410 bp,coding for 469 amino acids except 42 isolates were 466 amino acids with absence of 3 amino acids. BLAST analysis indicated that isolates shared the highest similarity with 97.62%-99.88% and 93.13%-99.79% to HA and NA genes in this study were from Vietnam and several provinces of China,and hosts were not identical,which suggested that the genetic of HA and NA genes was diversity.The nucleotide and amino acid similarity of Guangxi isolates intra were 93.0%-99.1% and 94.6%-99.1%,83.9%-98.2% and 86.1%-98.2%,respectively,which indicated that HA gene was more conserved,the similarity of Guangxi isolates with the reference strains of after 2016 was higher than strains of before,which manifested that the isolates were persisting evolution.The genetic analysis showed that HA and NA genes of Guangxi isolates belonged to G57 branch prevalent in China,the genetic distance of which and vaccine strains was farther.The evolutionary rate estimation of HA and NA genes was 3.99×10^-3and 4.59×10^-3substitution/sites/year,and the most recent common ancestor of which was 66.77 and 58.24 years,which suggested that the rate of HA gene was slower than NA gene.The recombination analysis of HA gene acquired from the chicken,duck and environment was positive signal,respectively.The major and minor parents strains were A/Beijing/1/2017 and A/quail/Hainan/250/2012.【Conclusion】 The HA and NA genes of Guangxi isolates with higher similarity to those of Vietnam and provinces of China,the genetic of them was diversity,and evolution progress was close to the dominant gene type G57,the recombination of HA genes was occurred between AIV and human-origin influenza strain,which could provide outbreak condition for novel subtype influence in future.

Key words: H9 subtype Avian influenza virus; genetic variation; evolutionary dynamics

CLC Number:

S852.65

LONG Feng, XIE Shouyu, CUI Pengfei, SHI Kaichuang, WEI Xiankai, FENG Shuping, QU Sujie, LU Wenjun, LI Jianfeng, YIN Yanwen, DENG Guohua. Genetic Evolutionary Dynamics Analysis of HA and NA Genes of H9 Subtype Avian Influenza Virus in Guangxi from 2016 to 2021[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1947-1958.

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Genetic Evolutionary Dynamics Analysis of HA and NA Genes of H9 Subtype Avian Influenza Virus in Guangxi from 2016 to 2021

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