2018年中国部分地区猪瘟病毒2.1d亚型新流行株的基因组特征分析

doi:10.16431/j.cnki.1671-7236.2020.08.025

Abstract

Abstract: In order to investigate the molecular epidemiology and genetic variation of classical swine fever virus (CSFV) in China,this study used the RT-PCR method to amplify E2 and NS5B genes of 350 samples suspected of CSFV infection that collected from Henan,Hebei,Shandong,Heilongjiang and Liaoning provinces in 2018,and the PCR products were sequenced and analyzed.The results showed that 21 of 350 samples were positive for CSFV detection and a total of 14 CSFV E2 gene sequences and 7 partial CSFV NS5B gene sequences were obtained.The complete E2 and partial NS5B gene sequences analysis showed that the 21 positive samples belonged to the 2.1d subtype CSFV that domestic epidemic in recent years.It found that there was no difference in homology between the newly 2.1d subtype CSFV and the earlier 2.1d subtype strains.These new 2.1d subtype CSFV isolates had the same molecular characteristics on the 6 amino acids (R³¹,S³⁴,W¹⁸²,K²⁰⁵,K³⁰³,A³³¹) of E2 gene,and 15 cysteines of E2 protein had no variation.The 2.1d subtype CSFV isolated in South Korean had three unique amino acids (N⁹⁷,K¹⁵⁹,R²⁰⁵) of E2 protein.The Korean strain YC11WB was an intermediate strain between 2.1b and 2.1d subtype CSFV,and the 2.1d subtype CSFV being prevalent in China and South Korea might be derived from the earlier domestic 2.1b subtype CSFV,respectively.This study confirmed that the relatively active and epidemic CSFV strains were still 2.1d subtype in China and neighboring countries in 2018,which providing a basis for scientific prevention and control of CSFV in China.

Key words: classical swine fever virus (CSFV); E2 gene; NS5B gene; 2.1d subtype; genetic variation; molecular characteristics

CLC Number:

S852.65⁺1

XU Hu, PAN Yan, SONG Mingxin. Genomic Characteristics of 2.1d Subtype Epidemic Strain of Classical Swine Fever in China in 2018[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2561-2570.

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Genomic Characteristics of 2.1d Subtype Epidemic Strain of Classical Swine Fever in China in 2018

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