China Animal Husbandry and Veterinary Medicine ›› 2022, Vol. 49 ›› Issue (12): 4866-4877.doi: 10.16431/j.cnki.1671-7236.2022.12.034

• Basic Veterinary Medicine • Previous Articles     Next Articles

Optimization of Foaming Performance of Compound Quaternary Ammonium Salt Foam Disinfectant and Evaluation of Its Killing Effect on Viruses

LIU Huan1,2, ZHANG Mengmeng2, CUI Yifang3, LIU Jing2, ZHANG Jingju2, CHEN Dengjin4, LI Xiubo2, LI Cun1, XU Fei2   

  1. 1. School of Animal Science and Animal Medicine, Tianjin Agricultural University, Tianjin 300384, China;
    2. National Standard Laboratory of Feed Drugs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    3. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100089, China;
    4. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
  • Received:2022-05-26 Online:2022-12-05 Published:2022-12-01

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Abstract: 【Objective】 The purpose of the test was to study the foaming performance of compound quaternary ammonium salt foam disinfectant, and evaluate the killing effect of the disinfectant on Newcastle disease virus (NDV) and Classical swine fever virus (CSFV), as well as the effect on the disinfection effect at different action concentrations and different action time.【Method】 The specific contents of benzalkonium bromide, decylmethyl ammonium bromide and decyl glucoside in the compound quaternary ammonium salt foam disinfectant were optimized by the response surface method.Suspension killing test and cell infection method were used to determine the inactivation rate of the disinfectant against NDV LaSota strain at dilution of 1:100, 1:200, 1:400, 1:500, 1:600, 1:800 and 1:1000 for 5, 10 and 15 min respectively.Suspension quantitative method was used to determine the inactivation rate of the disinfectant against CSFV Thiveral strain when the dilution was 1:200, 1:300, 1:400, 1:500 and 1:600 for 3, 5, 7, 10 and 15 min respectively.【Result】 When the content ratio of benzalkonium bromide, decylmethyl ammonium bromide and decyl glucoside in the compound quaternary ammonium salt foam disinfectant was 1.4%, 3.8% and 0.75%, the foam forming time reached 83 min.When the compound quaternary ammonium salt foam disinfectant was diluted up to 1:400 (about 137.5 mg/L in terms of total content), the minimum action time was 5 min, and the inactivation rate of NDV LaSota strain was 100%, and the inactivation rate of CSFV Thiveral strain reached 100% at 3 min, when the action time was extended to 15 minutes, and the inactivation rate of both viruses was stable at 100%.When the dilution multiple of compound quaternary ammonium salt foam disinfectant was less than 1:400, the killing effect on NDV LaSota strain was not good.When the dilution multiple was 1:500 (about 110 mg/L in terms of total content), 1:600 (about 91.67 mg/L in terms of total content), the action time was 10 min, and the inactivation rate of CSFV Theiveral strain reached 100%, and when the action time was extended to 15 min, the inactivation rate was stable at 100%.【Conclusion】 When the content of benzalkonium bromide, decylmethyl ammonium bromide and decyl glucoside in the compound quaternary ammonium salt foam disinfectant was 1.4%, 3.8% and 0.75%, the foaming effect was the best.The disinfectant had a good killing effect on NDV and CSFV, which provided reference and theoretical basis for the establishment of its disinfection effect on NDV and CSFV and its standardized use in farms, and also provided more possibilities for the evaluation of the environmental disinfection effect of livestock and poultry breeding sites.

Key words: foam disinfectant; Newcastle disease virus (NDV); Classical swine fever virus (CSFV)

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