猪瘟病毒E2基因的克隆及分子特性分析

doi:10.16431/j.cnki.1671-7236.2018.08.007

Abstract

Abstract:

To study the genetic variations of classical swine fever virus (CSFV) E2 gene in Sichuan province,we used RT-PCR method to amplify E2 gene and then cloned into pMD18-T vector,and strongly confirmed by PCR amplification,restriction digestion and sequencing,then the CSFV E2 gene was analyzed by bioinformatics softwares.The results showed that the CSFV E2 gene was 1 125 bp,and encoded a polypeptide of 375 amino acid residues.Moreover,the nucleobtide sequence of CSFV E2 had higher homology with its homologous protein of other CSFV strains GXWZ02,CSFV/2.1,HEBZ,YC11WB,SXYL2006 and 0406/CH/01/TWN in GenBank,the homologies were 98.3%,96.5%,94.7%,92.4%,92.1% and 90.9%,respectively,but it had lower homology with HCLV E2 gene (87.6%).The ENc value of E2 gene was 53.161,and there were distinctly differences of 33,25 and 25 codons frequency comparing CSFV E2 with E.coli,yeast and human,respectively.Besides,there were total 34 rare codons in CSFV E2 gene and serial rare codons being strung together.The results suggested that E2 gene was not obvious in codon preference,and was closest to eukaryotes in codon usage pattern.The nucleotides of E2 gene was partially mutated compared to vaccine strains,but the main antigenic domain didn't change.The results provided basic data for the further study of CSFV E2 function and the development of genetic engineering seedlings at later stages.

Key words: classical swine fever virus (CSFV); E2 gene; clone; molecular characterization analysis

CLC Number:

S852.65⁺1

LUO Dandan, LIAO Dangjin, YE Yonggang. Cloning and Molecular Characterization Analysis of E2 Gene of Classical Swine Fever Virus[J]. , 2018, 45(8): 2095-2103.

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Cloning and Molecular Characterization Analysis of E2 Gene of Classical Swine Fever Virus

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