延黄牛ALDH1A1基因CDS序列克隆及其在各组织中的表达差异研究

doi:10.16431/j.cnki.1671-7236.2019.01.020

Abstract

Abstract:

To explore the function of acetaldehyde dehydrogenase 1A1 (ALDH1A1) gene,16-month-old Yanhuang cattle were used as subjects,and total RNA were collected from the heart,liver,lung,kidney,stomach,duodenum,subcutaneous fat and longissimus dorsi after slaughter.According to the ALDH1A1 gene sequence published in GenBank(accession No.:NM_174239.2),primers were designed using Oligo 7.0 software,ALDH1A1 gene was amplified by RT-PCR,and the amplification product was connected into pMD18-T vector for clonging and sequencing.The complete CDS sequence of ALDH1A1 gene in Yanhuang cattle was obtained,the nucleotide sequences and its protein structures were analyzed using bioinformatics softwares.Then using the total RNA from different tissues of Yanhuang cattle as a template,the quantitative expression of ALDH1A1 gene in different tissues of Yanhuang cattle was detected by Real-time quantitative PCR.The results showed that the length of CDS sequence of ALDH1A1 gene was 1 506 bp,which encoded 501 amino acids.The homology of the ALDH1A1 gene sequence with bison and cattle was ≥ 99.7%,and its homology with cats and leopards were 89.4% and 89.6%,respectively,which was in accordance with species evolution rules.The molecular weight of ALDH1A1 was 54.805 ku,the isoelectric point was 7.16;A relatively strong hydrophilicity accounted for 86.4%;acidic and basic amino acids accounted for 11.4% and 12.2%,respectively.ALDH1A1 was soluble proteins,which was not secretory proteins,but there was no typical signal peptide cleavage site.There were 31 amino acid phosphorylation sites (score > 0.5).The secondary structure of ALDH1A1 protein in Yanhuang cattle contained alpha helix,extended chain,beta turn and random coil,which accounted for 42.12%,16.17%,8.18% and 33.53%,respectively,which were identical to the predicted structure of the tertiary structure of ALDH1A1 protein.Real-time quantitative PCR results showed that ALDH1A1 gene was extremely significantly expressed in liver,stomach,subcutaneous fat,duodenum and kidney tissues (P < 0.01),and significantly expressed in longissimus dorsi (P < 0.05) of Yanhuang cattle,respectively.This study results provided a reference for further research on the function of ALDH1A1 gene and the screening of meat gene in Yanhuang cattle.

Key words: Yanhuang cattle; ALDH1A1 gene; clone; expression difference; bioinformatics

CLC Number:

HU Zhongchang, CAO Yang, WU Jian, QIN Lihong, JIN Haiguo, ZHAO Yumin. Cloning of ALDH1A1 Gene CDS Sequence and Its Expression Difference of Different Tissues in Yanhuang Cattle[J]. , 2019, 46(1): 174-184.

References

[1] 吴德报,郝伟亮,杨立,等.ALDH1A1在舌癌及淋巴结中的表达及其临床意义[J].口腔医学研究,2017,33(3):315-319. WU D B,HAO W L,YANG L,et al.Expression and clinical significance of ALDH1A1 in tongue squamous cell carcinoma and lymph node[J].Journal of Oral Science Research,2017,33(3):315-319.(in Chinese)
[2] LIU Y,KURITA A,NAKASHIMA S,et al.3,4-dihydroxyphenylacetic acid is a potential aldehyde dehydrogenase inducer in murine hepatoma Hepa1c1c7 cells[J].Bioscience Biotechnologe Biochemistry,2017,81(10):1978-1983.
[3] NAGYMIHALY R,VEREB Z,FACSKO A,et al.Effect of isolation technique and location on the phenotype of human corneal stroma-derived cells[J].Stem Cells International,2017,2017:1-12.
[4] GOTO S,ONISHI A,MISAKI K,et al.Neural retina-specific Aldh1a1 controls dorsal choroidal vascular development via Sox9 expression in retinal pigment epithelial cells[J].Elife,2018,7(1):e32358.
[5] SOLOMON H,DINOWITZ N,PATERAS I S,et al.Mutant p53 gain of function underlies high expression levels of colorectal cancer stem cells markers[J].Oncogene,2018,37(12):1669-1684.
[6] KUMAR S,DOLLÉ P,GHYSELINCK N B,et al.Endogenous retinoic acid signaling is required for maintenance and regeneration of cornea[J].Experimental Eye Research, 2017,154:190-195.
[7] SGOBIO C,WU J,ZHENG W,et al.Aldehyde dehydrogenase 1-positive nigrostriatal dopaminergic fibers exhibit distinct projection pattern and dopamine release dynamics at mouse dorsal striatum[J].Scientific Reports,2017,7(1):5283.
[8] WANG B,ZHANG F,ZHANG H,et al.Alcohol intake aggravates adipose browning and muscle atrophy in cancer-associated cachexia[J].Oncotarget,2017,8(59):100411-100420.
[9] 左其生,张蕾,连超,等.脂代谢通路对鸡雄性生殖细胞分化的调控机制[J].中国农业科学,2015,48(22):4539-4550. ZUO Q S,ZHANG L,LIAN C,et al.The regulatory mechanism of the lipid metabolism pathways during male germ cell differentiation in chickens[J].Scienta Agricultura Sinica, 2015,48(22):4539-4550.(in Chinese)
[10] HAENISCH M,TREUTING P M,BRABB T,et al.Pharmacological inhibition of ALDH1A enzymes suppresses weight gain in a mouse model of diet-induced obesity[J].Obesity Research Clinical Practice,2017,10(17):30078-30079.
[11] SHEN Q,YASMEEN R,MARBOURG J,et al.Induction of innervation by encapsulated adipocytes with engineered vitamin A metabolism[J].Translational Research,2018,192:1-14.
[12] PICARD B,GAGAOUA M,AL-JAMMAS M,et al.Beef tenderness and intramuscular fat proteomic biomarkers:Muscle type effect[J].Peer-reviewed Scientific Mega Journal,2018,6:e4891.
[13] GAGAOUA M,BONNET M,ELLIESOURY M P,et al.Reverse phase protein arrays for the identification/validation of biomarkers of beef texture and their use for early classification of carcasses[J].Food Chemistry,2018,250:245-252.
[14] OLIVEIRA L J,MCCLELLAN S,HANSEN P J.Differentiation of the endometrial macrophage during pregnancy in the cow[J].Public Library of Science One,2010,5(10):e13213.
[15] 吴健,于永生,李娜,等.延黄牛和西门塔尔牛肉品质对比研究[J].吉林农业大学学报,2015,37(4):459-462. WU J,YU Y S,LI N,et al.Comparative study on meat quality traits of Yan Yellow cattle and Chinese Simmental[J].Journal of Jilin Agricultural University,2015,37(4):459-462.(in Chinese)
[16] 戢爽.肉牛脂肪组织和背最长肌脂代谢相关基因差异表达分析[D].长春:吉林大学,2013. JI S.Analysis of differentially expressed gene related to lipid metabolism between adipose tissues andlongissimus dorsi muscle in cattle[D].Changchun:Jilin University,2013.(in Chinese)
[17] TUNG J C,HUANG W C,YANG J C,et al.Auramine O,an incense smoke ingredient,promotes lung cancer malignancy[J].Environmental Toxicology,2017,32(11):2379-2391.
[18] NOURASHRAFEDDIN S,HOSSEINI R B.Gonadotropin regulation of retinoic acid activity in the testis[J].Acta Medical Iranica,2018,56(1):34-42.
[19] MINKINA A,LINDEMAN R,GEARHART M,et al.Retinoic acid signaling is dispensable for somaticdevelopment and function in the mammalian ovary[J].Developmental Biology,2017,424(2):208-220.
[20] MOREB J S,BAKER H V,CHANG L J,et al.ALDH isozymes downregulation affects cell growth cell motility and gene expression in lung cancer cells[J].Molecular Cancer,2008,7:87.
[21] TOMITA H,TANAKA K,TANAKA T,et al.Aldehyde dehydrogenase 1A1 in stem cells and cancer[J].Oncotarget,2016,7(10):11018-11032.
[22] YANG K,ADIN C,SHEN Q,et al.Aldehyde dehydrogenase 1 a1 regulates energy metabolism in adipocytes from different species[J].Xenotransplantation,2017,24:e12318.
[23] XIAO T,SHOEB M,SIDDIQUI M S,et al.Molecular cloning and oxidative modification of human lens ALDH1A1:Implication in impaired detoxification of lipid aldehydes A[J].Journal of Toxicology and Environmental Health,2009,72(9):577-584.
[24] 孙伟,李达,苏锐,等.绵羊YAP1基因全长cDNA克隆及生物信息学分析[J].中国农业科学,2013,46(8):1725-1735. SUN W,LI D,SU R,et al.Cloning and bioinformatics analysis of full-length cDNA sequence of YAP1 genein sheep[J].Scientia Agricultura Sinica,2013,46(8):1725-1735.(in Chinese)
[25] MANZER R,QAMAR L,ESTEY T,et al.Molecular cloning and baculovirus expression of the rabbitcorneal aldehyde dehydrogenase (ALDH1A1) cDNA[J].DNA Cell Biology,2003,22(5):329-338.
[26] WANG B,WANG Z,AVILA J M,et al.Moderate alcohol intake induces thermogenic brown/beigeadipocyte formation via elevating retinoic acid signaling[J].The Journal of the American Societies for Experimental Biology,2017,31(10):4612-4622.

Cloning of ALDH1A1 Gene CDS Sequence and Its Expression Difference of Different Tissues in Yanhuang Cattle

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Saiqiao, ZHAO Lu, ZHAI Zhenhan, ZHANG Binglei, JIA Wanhang, WANG Yuqin. Tissue Expression and Bioinformatics Analysis of miR-449a/b Precursor Sequences in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2175-2184.
[2]	WEI Mingbang, BIANBA Qiongda, XIAO Qingqing, DUAN Mengqi, CHAMBA Yangzom, SHANG Peng. Cloning,Bioinformatics and Expression Analysis of CDKN1B Gene in Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2196-2206.
[3]	LI Yongbin, YANG Huilin, SONG Zihao, ZHANG Yu, YANG Jin, MENG Jia, CUI Xiaozhen, LYU Xiaoling, ZHENG Mingxue, BAI Rui. Cloning and Bioinformatics Analysis of MIF Gene in Eimeria tenella [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2450-2459.
[4]	ZHANG Dianqi, MA Xinhao, YANG Zhimei, MEI Chugang, ZAN Linsen. Bioinformatics Analysis of PAI1 Gene and Its Effect on the Proliferation and Differentiation of Intramuscular Adipocytes in Qinchuan Beef Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1729-1741.
[5]	WANG Haorui, YU Baojun, YANG Jiayi, WEI Rong, FU Xi, WANG Chuanchuan, ZHANG Di, LI Desheng, CAI Zhengyun, GU Yaling, ZHANG Juan. Bioinformatics Analysis and Tissue Expression of DERA Gene in Jingyuan Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1764-1773.
[6]	CHEN Meiting, ZHENG Congsen, LIANG Zexian, LIN Qiaoer, CHANG Chuanzhe, ZHOU Jun, FENG Jun, LI Linlin, QIN Limei. Cloning and Bioinformatics Analysis of env Gene of Avian Leukosis Virus Subgroup J Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1785-1795.
[7]	PAN Pengcheng, LEI Zongquan, LI Xian, HU Xiangyun, QIN Qiantao, QIN Zhaoxian, GUAN Zhihui, CHEN Baojian, XIE Bingkun. Bioinformatics Analysis,Eukaryotic Expression Vector Construction and Tissue Expression of MYL2 Gene in Luchuan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1796-1806.
[8]	LIU Jialong, ZHANG Yiwen, ZHANG Yueran, SUN Qijuan, CHEN Jian, HE Lei, JIA Yanyan, DING Ke, YU Zuhua. Cloning,Prokaryotic Expression and Bioinformatics Analysis of ELAVL1 Gene in Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1807-1817.
[9]	ZHUO Na, ZHU Zhongwu, LI Jing, HU Shixiang, WANG Jianchang, LIN Hua, CHEN Chaolin, HAN Diangang, AI Jun, CHEN Peifu. Bioinformatics Analysis and Eukaryotic Expression of ORF2 Protein of Swine Hepatitis E Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1959-1970.
[10]	TAO Qinghua, HUANG Caiyun, CHEN Chao, LIU Wei, SUN Ningning, LI Ang. Cloning and Tissue Expression Difference Analysis of LIF Gene in White Muscovy Duck [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1296-1306.
[11]	FAN Bingfeng, LI Wen, LIU Lixiang, ZHAO Xiangyuan, SHAO Jing, LIN Lefeng, SUN Huimin, ZHANG Xulin, XU Baozeng. Cloning and Bioinformatics Analysis of AANAT Gene in Mink [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1307-1318.
[12]	JIA Xinyue, MA Jing, ZHANG Yanyan, MA Xun, WANG Zhengrong, BO Xinwen. Bioinformatics Analysis and Prokaryotic Expression of EGR-07243 and EGR-07243 Genes of Echinococcus granulosus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 847-858.
[13]	LI Dan, ZHANG Haisen, WANG Yiqun, GAO Dengke, ZHAO Hongcong, LI Chao, JIN Yaping, CHEN Huatao. Eukaryotic Expression Vector Construction,Bioinformatics and Tissue Expression Profiles Analysis of CRY1 Gene in Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 870-881.
[14]	SONG Xingchao, MENG Jinzhu, ZHAO Yuanyuan, WU Zhenyang, AN Qingming. Cloning and Bioinformatics Analysis of MyoG Gene Promoter Region Sequence in Guizhou White Goat [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 893-903.
[15]	ZHU Xihui, LI Yanlan, WANG Zhiwei, PANG Zheyu, LI Yubao, LIU Cheng, SI Zhenshu, LU Jianbiao, CAO Shengliang, XUE Xijuan. Whole Genome Sequencing of a Resistant Riemerella anatipestifer Strain and Analysis of Antibiotic Resistance and Genetic Evolution [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1140-1149.