1株J亚群禽白血病病毒env基因克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2023.05.006

Abstract

Abstract: 【Objective】 The purpose of this study was to characterize the envelope protein (env) of an Avian leukosis virus (ALV) strain by bioinformatics method.【Method】 The serum samples from suspected avian leukosis ckickens were cultured in chicken fibroblast (DF-1),and the subgroups of ALV was identified by ELISA and Real-time quantitative PCR.The PCR products of env gene were sequenced and spliced by SeqMan.The env gene sequence was compared with several ALV subpopulations both domestically and abroad,and the genetic evolution analysis was performed.The env proteins were analyzed with a variety of bioinformatics software,including physicochemical properties,hydrophilicity and hydrophobicity,transmembrane domain,signal peptide,glycosylation site,phosphorylation site,secondary structure,tertiary structure,acetylation site,epitope and B cell epitopes prediction.【Result】 The full length of env gene of ALV-J strain was 1 719 bp,and GenBank accession No.:OP837418.It was located in the same evolutionary branch with ALV-J subgroup,and the similarity was 89.3%-94.8%.The env protein had two transmembrane domains and was a hydrophilic and unstable protein composed of 572 amino acids with a predicted instability coefficient of 43.49 and a total average hydrophilicity of -0.201.The env protein was a non-secreted protein,without signal peptide,with 17 N-glycosylation modification sites,77 O-glycosylation modification sites,42 phosphorylation modification sites,21 potential epitope sites and 9 linear B cell epitopes with more than 10 consecutive bases.In the secondary structure of the env protein,the proportion of randorn coil was the largest (38.29%).【Conclusion】 The env gene was a key gene that caused genetic variation in ALV, and its encoded envelope protein env was unstable, with multiple protein modification sites and antigenic epitopes.

Key words: Avian leukemia virus (ALV); subgroup J; env gene; bioinformatics

CLC Number:

S858.31

CHEN Meiting, ZHENG Congsen, LIANG Zexian, LIN Qiaoer, CHANG Chuanzhe, ZHOU Jun, FENG Jun, LI Linlin, QIN Limei. Cloning and Bioinformatics Analysis of env Gene of Avian Leukosis Virus Subgroup J Strain[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1785-1795.

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Cloning and Bioinformatics Analysis of env Gene of Avian Leukosis Virus Subgroup J Strain

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