Marc145细胞源EDC3基因的克隆及其生物信息学分析

doi:10.16431/j.cnki.1671-7236.2018.08.008

Abstract

Abstract:

In order to obtain the sequence of EDC3 gene from Marc145 cell,and analyze the sequence characteristics of EDC3 gene and the structure and function of EDC3 encoding protein,EDC3 gene from Marc145 cells was amplified and cloned by RT-PCR method.The nucleotide and amino acid sequences of EDC3 gene were analyzed by DNAStar software,the homology was analyzed by BLAST,and the phylogenetic tree was constructed.The secondary and tertiary structures,B cell epitopes,transmembrane domain and signal peptide of EDC3 protein were predicted by bioinformatics softwares.The results showed that the length of EDC3 gene was 1 527 bp,which encoded 507 amino acids.The CDS sequence and amino acid sequence of EDC3 gene from Marc145 cell shared 91.5% to 99.2% and 95.3% to 99.6% identity with Chlorocebus sabaeus,Sus scrofa,Pan paniscus,Papio anubis,Macaca mulatta,Homo sapiens,Orcinus orca,Ovis aries,Ailuropoda melanoleuca,Equus caballus and Loxodonta africana,respectively,the shared lowest homology was 81.2% and 88.8% with Gallus gallus.The results of phylogenetic tree showed that EDC3 gene from Marc145 cell was closely relative with Chlorocebus sabaeus,followed by primates.The alpha helix and radom coil were dominant in secondary and tertiary structures,which was 23.38% and 47.35%,respectively.The results predicted that the protein had multiple B cell dominant antigen epitopes,and there was no transmembrane domain and signal peptide region.The results might provide references for further research on the function of EDC3 gene from Marc145 cell.

Key words: Marc145 cell; EDC3 gene; clone; bioinformatics analysis

CLC Number:

LI Changhong, WEN Guilan, ZHANG Shengbo, CHEN Shaopin, LIN Hanqing, XU Li, GUAN Guodan, WANG Desheng, JI Xinqin, WEN Ming, ZHOU Bijun, CHENG Zhentao. Cloning and Bioinformatics Analysis of EDC3 Gene from Marc145 Cells[J]. , 2018, 45(8): 2104-2112.

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Cloning and Bioinformatics Analysis of EDC3 Gene from Marc145 Cells

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