麝鼠香腺Elovl1基因生物信息学分析及表达研究

doi:10.16431/j.cnki.1671-7236.2022.04.001

Abstract

Abstract: 【Objective】 This study was aimed to determine the nucleotide sequence of elongase of verylong chain fatty acid 1 (Elovl1) gene in muskrat and analyze its bioinformatics,and explore its role in the development and secretion of muskrat scent gland.【Method】 The total RNA was extracted from adult male muskrat’s fragrant gland by TRIzol method,the sequence of Elovl1 gene in muskrat scent gland was obtained by transcriptome sequencing.The CDS sequence of Elovl1 gene and its multiple transcripts of different species of rodents were downloaded in GenBank database,and phylogenetic tree was constructed.The physicochemical property and structure of Elovl1 gene encoded protein were predicted and analyzed using online tools.The transcription level of Elovl1 gene was analyzed in scent gland tissue during the developmental period of adult male muskrat by Real-time quantitative PCR.【Result】 There were two transcripts of Elovl1 gene in muskrat scent gland,which could encode two different proteins,with lengths of 313 and 279 amino acids,respectively.Both transcripts of Elovl1 gene were expressed in muskrat scent gland from the end of February to the end of September,and the overall expression level showed a trend of up-regulation first and then down-regulation.The two proteins encoded by Elovl1 gene were bothstable lipophilic and non-secreted proteins,with 7 transmembrane helix regions,and their subcellular locations were mainly in the endoplasmic reticulum.In rodents,the genetic evolution of Elovl1 gene was basically the same as that of species.Circetidae and Muridae were closely related,Ondatra zibethicus and Arvicola amphibius were closely related.The different transcripts of Elovl1 gene of the same species were relatively conserved,both grouped by species and generally had higher confidence values,with the exception of Marmota.【Conclusion】 The expression pattern of Elovl1 gene was consistent with the development,atrophy and secretion period of muskrat scent gland,suggesting that Elovl 1 was related to muskrat scent secretion,and should be involved in the development of scent gland cells.

Key words: muskrat; scent gland; Elovl1 gene; expression; bioinformatics analysis

CLC Number:

LEI Xing, LI Tianfeng, LI Jiahao, MAN Xin, BAI Suying. Bioinformatics Analysis and Expression of Elovl1 Gene in Muskrat Scent Gland[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1201-1212.

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Bioinformatics Analysis and Expression of Elovl1 Gene in Muskrat Scent Gland

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