阳原驴MSTN基因生物信息学分析及组织表达研究

doi:10.16431/j.cnki.1671-7236.2022.08.007

Abstract

Abstract: 【Objective】 The purpose of this study was to analyze the CDS sequence of myostatin (MSTN) gene and its expression in different growth stages and different tissues of Yangyuan donkeys.【Method】 Blood samples and various tissue samples were collected from Yangyuan donkeys aged 6,12,18 and 24 months old,genomic DNA and total RNA from different tissues were extracted. The CDS sequence of MSTN gene in Yangyuan donkeys was amplified by PCR and analyzed by bioinformatics.Real-time quantitative PCR was used to detect the expression of MSTN gene in longissimus dorsi muscle,leg muscle of Yangyuan donkeys at different growth and development stages, and different organizations (heart,liver,spleen,lung,kidney) of 18 months old Yangyuan donkeys.【Result】 The CDS sequence of MSTN gene in Yangyuan donkeys was 1 128 bp and encoded 375 amino acids,which had the highest similarity of nucleotide sequences (99.9%) with Equus caballus.The encoded protein was an unstable hydrophilic protein,containing transforming growth factor β (TGF-β)superfamily domain,no transmembrane region,1 signal peptide region,6 O-glycosylation sites,1 N-glycosylation site and 34 phosphorylation sites,mainly distributed in nucleus (39.1%),and the proportion of random coil in the secondary structure was the highest (50.93%).The expression of MSTN gene in longissimus dorsi muscle and leg muscle of Yangyuan donkeys at 18 months old were significantly higher than that of 6,12 and 24 months old (P<0.05).MSTN gene was expressed in different tissues of 18 months old Yangyuan donkeys.The expression in longissimus dorsi muscle was significantly higher than that in other tissues (P<0.05),followed by leg muscle,lung,spleen,kidney and liver,and the expression in heart was the lowest.【Conclusion】 The CDS sequence of MSTN gene in Yangyuan donkeys was successfully amplified.The expression of MSTN gene in longissimus dorsi muscle and leg muscle of Yangyuan donkeys at different growth and development stages was the highest at 18 months old,and the expression of longissimus dorsi muscle and leg muscle was higher in different tissues at 18 months old.The results provided a reference for further exploring the mechanism of MSTN gene in the growth and development of skeletal muscle in Yangyuan donkeys.

Key words: Yangyuan donkeys; MSTN gene; bioinformatics analysis; tissue expression

CLC Number:

FU Yongzhi, MA Xiaojun, WANG Maohan, LI Zhe, CHEN Zhiyuan, QUAN Wei, ZHAO Han, SU Yongmei, WU Jianhua, ZHU Wenjin. Bioinformatics Analysis and Tissue Expression of MSTN Gene in Yangyuan Donkeys[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2910-2919.

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Bioinformatics Analysis and Tissue Expression of MSTN Gene in Yangyuan Donkeys

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