不同品种猪PBD-124基因多态性及差异表达研究

doi:10.16431/j.cnki.1671-7236.2022.06.021

Abstract

Abstract: 【Objective】 The CDS region of porcine beta-defensin-124 (PBD-124) gene was cloned and its polymorphism was explored.The expression of PBD-124 gene in different breeds of pigs and different tissues of the same kind of pigs was analyzed.【Method】 The CDS region of PBD-124 gene was amplified by RT-PCR and cloned.PCR-RFLP was used to detect the polymorphism of PBD-124 gene (Bln Ⅰ) in Large White, Min and wild hybrid pigs.The expression difference of PBD-124 gene in liver, spleen and blood of different breeds of pigs was detected by Real-time quantitative PCR.【Result】 The CDS region of PBD-124 gene was successfully cloned, with a length of 423 bp and a total of 140 amino acids.Sequencing results showed that there were two mutation sites of c.257 G>A and c.263 T>G.Because there was linkage between the two mutation sites, only the first mutation site was digested.Three genotypes of GG, GA and AA were detected in Large White pigs, while only two genotypes of GA and AA were detected in Min and wild hybrid pigs.AA genotype was the dominant genotype in three pig populations.The genotype frequency of Large White, Min and wild hybrid pigs were 0.5261, 0.9412 and 0.6452, respectively, and three pig populations were in Hardy-Weinberg equilibrium (P>0.05).The polymorphism of three pig populations was not high.Large White pigs were in moderate polypeptide (0.25<PIC<0.5), while Min and wild hybrid pigs were in low polypeptide (PIC<0.25).Real-time quantitative PCR results showed that PBD-124 gene was expressed in spleen, liver and blood of Large White, Min and wild hybrid pigs, and there were expression differences.【Conclusion】 The length of PBD-124 gene CDS was 423 bp, and encoded 140 amino acids.Compared with the reference sequence, two SNP mutation sites were found, which did not cause amino acid mutation.The polymorphism of three pig populations was not high.The expression of PBD-124 gene was different in different breeds of pigs and different tissues of the same kind of pigs.

Key words: Large White pigs; Min pigs; wild hybrid pigs; PBD-124 gene; PCR-RFLP; tissue expression

CLC Number:

S813.3

GAO Beiyao, LIU Yanguang, JIA Qi, LIU Jianqiang, LUO Xinhui, ZHANG Lichun. Study on Polymorphism and Differential Expression of PBD-124 Gene in Different Breeds of Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2208-2215.

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Study on Polymorphism and Differential Expression of PBD-124 Gene in Different Breeds of Pigs

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