静原鸡let-7a-5p的靶基因预测及组织表达分析

doi:10.16431/j.cnki.1671-7236.2022.10.002

Abstract

Abstract: 【Objective】 This study was aimed to understand the biological information of let-7a-5p,and initially explore its functional role in the muscle tissue of Jingyuan chickens.【Method】 The mature sequence of let-7a-5p in Jingyuan chickens obtained by transcriptome sequencing was conservatively analyzed by the bioinformatics method.The target genes of let-7a-5p were predicted by miRDB,TargetScan,and miRanda online softwares,and their intersection was used for GO function and KEGG pathway enrichment analysis.Real-time quantitative PCR was used to detect the expression of let-7a-5p in tissues of female and male in Jingyuan chickens.【Result】 The results showed that the mature sequence of let-7a-5p was highly conserved among species,and 38 gene sets were predicted.The GO items with rich target genes in biological process,cellular composition and molecular function were cellular process,biological regulation,cell,binding,and catalytic activity.Target genes were significantly enriched in ketoglycan biosynthesis,the role of AGE-RAGE signal pathway in diabetic complications,ubiquitin-mediated proteolysis,TGF-β signal pathway and vasopressin-regulated water reabsorption signal pathway,and the most enriched was metabolic pathway,the role of AGE-RAGE signal pathway in diabetic complications,ubiquitin-mediated proteolysis and MAPK signal pathway.The interaction of let-7a-5p-target genes mainly inhibited the expression or degraded the target mRNA by acting on its 3'-UTR.Tissue expression results revealed that let-7a-5p was expressed in heart,liver,spleen,lung,kidney,chest muscle, and leg muscle tissues of female and male in Jingyuan chickens,and the expression was higher in chest muscle and leg muscle.【Conclusion】 let-7a-5p might regulate the expression of target genes and participate in the muscle growth and development of Jingyuan chickens,which provided a theoretical basis for further exploring the function and mechanism of let-7a-5p.

Key words: Jingyuan chickens; let-7a-5p; target gene; bioinformatics; tissue expression profile

CLC Number:

DENG Zhanzhao, YU Baojun, CAO Guowei, ZHANG Juan, SHI Yonghong, HU Weilon, QIU Xiuling. Target Gene Prediction and Tissue Expression Analysis of let-7a-5p in Jingyuan Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3704-3712.

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Target Gene Prediction and Tissue Expression Analysis of let-7a-5p in Jingyuan Chickens

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