CRISPR/Cas9系统在寄生虫基因组编辑中的应用研究进展

doi:10.16431/j.cnki.1671-7236.2022.10.003

Abstract

Abstract: Gene editing is a modern biological technology that relies on endonuclease for directional modification of target genes,it can achieve the addition and deletion of specific fragments and the insertion,deletion and replacement of specific bases.The CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/associated nuclease 9) system is an acquired immune system found in bacteria and archaea to resist the invasion of foreign plasmids and phages.After modification,it has become a novel genome editing tool.Because of its advantages of simple operation,low cost and high cutting efficiency,it is widely used in genome editing of various animals,plants and microorganisms.CRISPR/Cas9 system can be used to analyze and verify gene functions,study genetic breeding and screen drug targets and vaccine molecules on the basis of gene editing,so as to reduce the pathogenicity of pathogenic microorganisms,enhance the disease resistance of animals and plants,and treat genetic diseases,viral infections and tumors.Due to the complex life history of parasites,larger genome and more complex structure compared with bacteria and virus,and lack of effective research tools,relevant research progress is slow.However,the emergence of CRISPR/Cas9 gene editing technology provides a new technical means for gene function research.In this review,the discovery of CRISPR/Cas9 system and its application in parasite genome editing were reviewed,and the application status and development of CRISPR/Cas9 system were summarized and prospeced.

Key words: gene editing; CRISPR/Cas9 system; parasite

CLC Number:

S852.7

MA Jing, ZHANG Yanyan, JIA Xinyue, MA Xun, WANG Zhengrong, BO Xinwen. Research Progress of CRISPR/Cas9 System in Parasite Genome Editing[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3713-3725.

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Research Progress of CRISPR/Cas9 System in Parasite Genome Editing

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