CRISPR/Cas9系统在家禽中应用研究进展

doi:10.16431/j.cnki.1671-7236.2020.01.017

Abstract

Abstract: The CRISPR/Cas system is an adaptive immune system of bacteria and archaebacteria,which includes clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas).Based on CRISPR/Cas9 (CRISPR-associated protein 9,Cas9) system,sgRNA (single guide RNA) could be designed and genome editing could be operated in specific location.As agricultural economic animals,poultry could produce eggs and meat quickly and efficiently.It's an important source of protein,and could be used as the valuable biological model for vertebrate development.However,as the oviparous animals,the fertilized eggs of poultry are closely bound to the surface of the yolk,and the unfertilized eggs are difficult to extract.So it's very hard to operate the specific genetic modification on poultry eggs,which prevents the research progress of functional genes and transgenic poultry.CRISPR/Cas9 system has the advantages of high targeting,easy operation and with wide range of application,and has greatly promoted the research progress on poultry and soon become one of the most effective tools in poultry science.This paper reviews the progress of CRISPR/Cas9 research on poultry cells,growth and development,gonadal differentiation,diseases,embryo editing and transgenic poultry preparation,which would provide theoretical basis and practical reference for the future research and application of CRISPR/Cas in poultry.

Key words: CRISPR/Cas9 system; poultry; genome editing; transgenic

CLC Number:

S813.3

ZHANG Lei, ZHANG Haibo, ZHANG Jingqi, ZHANG Yang, CHANG Guobin, CHEN Guohong, WANG Jian. Research Progress on Application of CRISPR/Cas9 System in Poultry[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 140-147.

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Research Progress on Application of CRISPR/Cas9 System in Poultry

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