CRISPR/Cas9基因编辑技术在绵羊中的应用研究进展

doi:10.16431/j.cnki.1671-7236.2022.11.003

Abstract

Abstract: With the rapid development of genome editing tools, many significant breakthroughs have been achieved in numerous fields.Among the current gene editing tools, the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9)-based system is the most widely used in researches.CRISPR/Cas9 and its derived systems, base editor (BE) and prime editor (PE), enable researchers to easily and efficiently accomplish desired modification in genomes, such as deletion, insertion and substitution.CRISPR/Cas9-based systems have great potential in producing animals with specific genetic characteristics.Sheep are economically important livestock which has great potential as ideal large animal models for genome engineering.CRISPR/Cas9-based systems have been utilized in sheep genome engineering for generating superior agricultural breeds, expression of therapeutic agents in mammary glands, and developing animal models for human disease and regenerative medicine research.Starting from the principle of CRISPR/Cas9-based systems, the author focused on the specific process of genome editing using CRISPR/Cas9-based systems, and summarized the application of CRISPR/Cas9-based systems in research and production of sheep.

Key words: CRISPR/Cas9-based systems; genome editing; sheep

CLC Number:

S813.3

XU Xin, LIU Mingjun. Research Progress on Application of CRISPR/Cas9 Genome Editing Systems in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4129-4138.

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Research Progress on Application of CRISPR/Cas9 Genome Editing Systems in Sheep

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