大白猪ZnT基因家族生物信息学分析及组织表达研究

doi:10.16431/j.cnki.1671-7236.2022.11.002

Abstract

Abstract: 【Objective】 The aim of this study was to analyze the biological characteristics of solute carrier 30A(ZnT) gene in Yorkshire pigs, and detect the expression of ZnT gene in different tissues of pigs, so as to provide a scientific basis for the study on the regulation of zinc metabolism by ZnT gene family.【Method】 Various bioinformatics software were used to analyze the sequence of ZnT gene family (ZnT1-ZnT10), including motif, conserved domain, transmembrane domain, evolutionary tree and protein interaction.Real-time quantitative PCR was used to detect the expression differences of ZnT gene family in heart, liver, spleen, lung, kidney, ovary and breast in Yorkshire pigs.【Result】 ZnT1-ZnT10 genes were located on 8 chromosomes of pigs, the molecular weight were 41.61-85.30 ku, and the isoelectric points were 6.27(ZnT1)-9.30(ZnT6).In the ZnT gene family, they all belonged to stable proteins except for ZnT3, contained 6-layer transmembrane domains except for ZnT5 and ZnT9, and the sequence of motifs were the same except for ZnT6 and ZnT9.Cation-efflux was a common conservative domain of ZnT gene family.ZnT6 and ZnT2, ZnT3, ZnT7 and ZnT9 proteins had the highest interrelationship, ZnT1 and ZnT3 proteins had the highest interrelationship, and ZnT8 had a distant relationship with other family members.The sequences of ZnT gene family were relatively conserved during mammalian evolution.The 10 genes of ZnT gene family had higher expression in lung and lower expression in heart of Yorkshire pigs, except for ZnT5, ZnT7 and ZnT9 genes, the other members of ZnT gene family were almost not expressed in muscle.【Conclusion】 ZnT family belonged to transmembrane structural proteins, and the motifs were basically in the same order.Cation-efflux was a common conserved domain of ZnT gene family.Most members of ZnT gene family had the highest expression in lung, and the low expression in heart and muscle of Yorkshire pigs.

Key words: Yorkshire pigs; ZnT gene family; bioinformatics analysis; tissue expression

CLC Number:

LI Yueyue, LYU Dongling, LIU Xiaoxiao, TIAN Weilong, LIANG Liang, CHEN Kuirong, CHENG Feng, LAN Ganqiu, LIANG Jing. Bioinformatics Analysis and Tissue Expression of ZnT Gene Family in Yorkshire Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4119-4128.

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Bioinformatics Analysis and Tissue Expression of ZnT Gene Family in Yorkshire Pigs

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