CRISPR/Cas9技术在猪、鸡中的应用研究进展

doi:10.16431/j.cnki.1671-7236.2022.12.016

Abstract

Abstract: Gene editing relies on nucleases to generate DNA double-strand breaks at specific sites in the genome, thereby triggering the cell’s own DNA damage repair mechanism to achieve site-specific modification of target gene sequences.Clustered regularly interspersed short palindromic repeats (CRISPR) and its related protein 9 (Cas9) system play an important role in agriculture and gene therapy research as a third-generation gene editing technology.Compared with the gene editing method of transcription activator-like effector ribozyme, zinc finger ribozyme, it can target any site of gene to cause DNA double-strand break, achieve precise knockout of target gene or insert foreign fragment, and can quickly efficiently modifying the genome, including gene knockout, knockin, repression, activation, etc., is one of the most widely used gene editing tools.The emergence of CRISPR/Cas9 technology has revolutionized the research status of life science, medicine and genetics.In recent years, the use of CRISPR/Cas9 technology to modify animal genomes has opened a new era of molecular breeding of livestock and poultry, which not only greatly promoted the development of modern livestock and poultry breeding technology, but also made special contributions to human medical research, especially in pigs and chickens.Taking pigs and chickens as objects, the author reviewed the recent research progress on the CRISPR/Cas9 system in allogeneic organ transplant donors, biological models of human diseases, preparation of disease-resistant breeding materials, and genome-wide high-throughput screening, and how to use CRISPR technology to quickly and simply produce gene editing pigs and chickens, so as to provide a reference basis for promoting CRISPR technology to prepare other gene editing animals.

Key words: CRISPR/Cas9 technology; pigs; chickens; gene editing; gene function

CLC Number:

LI Xiaojiao, HE Yanhua, ZHU Xinyu, ZOU Xian, LUO Chenglong. Research Progress on Application of CRISPR/Cas9 Technology in Pigs and Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4665-4673.

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Research Progress on Application of CRISPR/Cas9 Technology in Pigs and Chickens

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