北京黑猪MYH3和MYH13基因多态性与肉质性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.09.017

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the effects of genetic variation of myosin heavy chain 3 (MYH3) and myosin heavy chain 13 (MYH13) on meat quality traits in pigs.【Method】 The meat quality traits (intramuscular fat (IMF), moisture, drip loss, pH, meat color (L^*, a^* and b^* values)) of longissimus dorsi muscle samples in Beijing Black pigs were measured.The promoter and CDS regions of MYH3 and MYH13 genes were genotyped by PCR and Sanger sequencing.The SNPs associated with meat quality traits were determined by covariance analysis using sex, field and age as covariates, the expression of MYH3 and MYH13 genes were detected by Real-time quantitative PCR.【Result】 A total of nine SNPs were screened in this study.There was a missense mutation (c.5782 G>C) in CDS region of MYH3 gene, which was significantly correlated with IMF content (P<0.05), the expression of MYH3 gene was positively correlated with IMF content.There were four SNPs in CDS region of MYH13 gene, of which two SNPs (c.1923 G>A and c.1308 G>A) were significantly correlated with meat color a^*value, one SNP (c.963 G>A) was significantly correlated with drip loss, one SNP (c.237 G>T) was significantly associated with meat color L^* value (P<0.05).There were four SNPs in promoter region of MYH13 gene, two SNPs (rs699287502 and rs318639161) were significantly associated with meat color L^* value, two SNPs (rs321315318 and rs330770991) were significantly related to drip loss (P<0.05), and the expression of MYH13 gene was negatively correlated with drip loss.【Conclusion】 One SNP of MYH3 gene was significantly associated with IMF, and its gene expression was positively correlated with IMF content.Three SNPs of MYH13 gene were significantly associated with drip loss, the expression of two SNPs of MYH13 gene promoter region was negatively correlated with drip loss.Three SNPs of MYH13 gene were significantly associated with meat color L^* value, two SNPs of MYH13 gene were significantly associated with meat color a^* value.These SNPs could be used as candidate gene functional loci for meat quality traits in Beijing Black pigs.

Key words: Beijing Black pigs; MYH3 gene; MYH13 gene; meat quality traits

CLC Number:

S813.3

YANG Man, LIU Hai, ZHANG Run, HU Ziping, NIU Naiqi, WANG Lixian, ZHANG Longchao. Association Analysis of Polymorphism of MYH3 and MYH13 Genes with Meat Quality Traits in Beijing Black Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3428-3437.

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Association Analysis of Polymorphism of MYH3 and MYH13 Genes with Meat Quality Traits in Beijing Black Pigs

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