›› 2017, Vol. 44 ›› Issue (6): 1778-1783.doi: 10.16431/j.cnki.1671-7236.2017.06.028

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Polymorphism of MyoG Gene Exon 1 and Its Association Analysis with Meat Quality Traits in Sheep

BAI Jun-yan, YANG You-bing, WANG Yu-qin, PANG You-zhi, WANG Xu, YANG Shuai, WANG Huan-ling   

  1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China
  • Received:2016-10-17 Online:2017-06-20 Published:2017-06-28

Abstract:

Myogenin (MyoG) gene plays a central regulatory role in the process of muscle cell differentiation, which directly affects meat production capacity of animal. In this study, 6 sheep varieties including Large-tail Han sheep, Small-tail Han sheep, Yuxizhiwei sheep, Lanzhou fat-tailed sheep, Mongolia sheep and Tong sheep were selected as experimental materials to detect the polymorphism of MyoG gene exon 1 by non denaturing polyacrylamide gel electrophoresis, and analyze the association analysis between the polymorphism of MyoG gene exon 1 and meat quality traits in sheep. The results showed that 2 alleles (A, B) and 3 genotypes (AA, BB, AB) were detected in MyoG gene exon 1 of six sheep populations. The A allele frequency in Small-tail Han sheep, Large-tail Han sheep, Yuxizhiwei sheep, Lanzhou fat-tailed sheep, Mongolia sheep and Tong sheep were 0.5167, 0.2500, 0.4375, 0.6500, 0.5750 and 0.7125, respectively, the B allele frequency of six sheep populations were 0.4833, 0.7500, 0.5625, 0.3500, 0.4250 and 0.2875, respectively. MyoG gene exon 1 mainly affected the moisture and color of mutton, the moisture content of BB genotype was significantly higher than that of AB genotype (P< 0.01) and AA genotype (P< 0.05). The color of AB genotype was significantly higher than that of BB genotype (P< 0.01) and AA genotype (P< 0.05).

Key words: sheep; MyoG gene; meat quality traits; association analysis

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