山羊ZBP1基因多态性及与产羔性能的关联分析

doi:10.16431/j.cnki.1671-7236.2023.01.017

Abstract

Abstract: 【Objective】 The purpose of this experiment was to study the association between single nucleotide polymorphism (SNP) in 3'-UTR, intron 1 and exon 7 of Z-DNA binding protein 1 (ZBP1) gene and lambing performance in goats, and provide new genetic markers for molecular breeding of high fertility goats.【Method】 Macheng Black goats, Black headed goats and Boer goats were selected as research samples, the blood samples were collected and DNA was extracted.Primers were designed according to the location information of SNP selected from transcriptome data in ZBP1 gene sequence of goats.Multiple single base extension SNP typing technique (SNaPshot) was used to analyze the genetic diversity of ZBP1 gene SNP.General linear model (GLM) was used to analyze the relationship between SNP of ZBP1 gene and lambing performance of 3 goat breeds.【Result】 There were 12 potential SNPs of ZBP1 gene in goats, of which 7 SNPs (g.9734 A>G, g.9772 T>C, g.352 C>T, g.955 C>T, g.1880 G>A, g.2566 T>C and g.7919 G>A) were polymorphic.There were 2 genotypes of AA and GA at g.9734 A>G in Macheng Black goats population, the other 6 SNPs showed 3 genotypes including CC(AA), CT(GA) and TT(GG) in Macheng Black goats, Boer goats and Black headed goats.g.9734 A>G and g.9772 T>C showed low polymorphism in Boer goats population (PIC<0.25), g.9734 G>A and g.7919 G>A showed low polymorphism in Macheng Black goats population (PIC<0.25), while g.9772 T>C, g.352 C>T, g.955 C>T, g.1880 G>A and g.2566 T>C showed moderate polymorphism in Black headed goats population (0.25<PIC<0.5), g.9734 A>G, g.352 C>T and g.7919 G>A showed low polymorphism in Black headed goats (PIC<0.25).The chi-square fitness test results show that g.9734 A>G, g.9772 T>C and g.352 C>T were in Hardy-Weinberg equilibrium in 3 goat populations (P>0.05), g.2566 T>C in Boer goats and Macheng Black goats population was in Hardy-Weinberg equilibrium (P>0.05).Correlation analysis results showed that g.9772 T>C was significantly associated with the first litter sizes in Boer goats (P<0.05), g.352 C>T and g.7919 G>A were significantly or extremely significantly associated with the overall litter sizes in Boer goats(P<0.05;P<0.01).g.352 C>T were extremely significantly associated with the overall litter sizes in Black head goat (P<0.01).g.9772 T>C was significantly associated with the first litter sizes in Macheng Black goats, g.9734 A>G and g.9772 T>C were significantly associated with the overall litter sizes in Macheng Black goats (P<0.05).Linkage disequilibrium (LD) results showed that there were strong linkage disequilibrium between g.352 C>T, g.2566 T>C, g.7919 T>C and g.9734 A>T in Boer goats, (D'>0.85, r²=1). g.352 C>T and g 2566 T>C, and g.2566 T>C, g.9734 A>T and g.9772 T>C in Black headed goats were in strong linkage equilibrium (D'>0.83, r²=1).There were strong linkage disequilibrium between g.352 C>T and g.9772 T>C in Macheng Black goats (D'>0.87, r²=1).【Conclusion】 There were 7 SNPs were found in this study, 5 fully linked SNPs were idetified on ZBP1 gene, which indicated that the 4 SNPs (g.352 C>T, g.7919 G>A, g.9772 T>C and g.9734 A>G) could be used as potential candidate genetic markers for lambing performance in the breeding process of specific goat breeds, it provided reference data for molecular marker saaisted breeding of goats.

Key words: goats; ZBP1 gene; polymorphism; lambing performance; association analysis

CLC Number:

S813.3

LIU Zelin, YANG Juan, XU Mingzhu, ZHANG Nian, XIONG Qi, LIU Jingbo, TAO Hu. Association Analysis Between Polymorphisms of ZBP1 Gene and Lambing Performance in Goats[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 161-173.

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Association Analysis Between Polymorphisms of ZBP1 Gene and Lambing Performance in Goats

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