China Animal Husbandry and Veterinary Medicine ›› 2022, Vol. 49 ›› Issue (9): 3453-3464.doi: 10.16431/j.cnki.1671-7236.2022.09.019

• Genetics and Breeding • Previous Articles     Next Articles

Difference Analysis of Metabolites in Testis of Guanzhong Dairy Goats at Different Developmental Stages

WANG Guang, ZOU Jiahao, ZHANG Yongtao, LI Dexian, LI Xueqing, YU Mengqi, CHEN Lu, YUAN Yuxin, LI Guang   

  1. Technical Innovation Laboratory of Dairy Sheep Industry, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China
  • Received:2022-03-04 Online:2022-09-05 Published:2022-08-24

Abstract: 【Objective】 The purpose of this study was to explore the metabolic mechanism of testis development in Guanzhong dairy goats from the perspective of metabolomics.【Method】 Six 1 month old weaned male and six 24 months old physically mature male of Guanzhong dairy goats were selected to collect testis tissue, and the testis tissues were chemically detected by liquid chromatography mass spectrometry (LC-MS) technology.The chemical detection, comparison and identification of different metabolites in two groups were carried out through the combination of multi-dimensional and single dimensional analysis.The potential key pathways in developmental metabolism of Guanzhong dairy goats were screened out using the enrichment differential metabolite analysis.【Result】 A total of 334 differential metabolites were screened out in testis of Guanzhong dairy goats at 1 and 24 months old, of which 137 were significantly upregulated and 197 were significantly downregulated.The top 36 differential metabolites were compared and identified, which were classified into 4 categories:Lipids and lipid-like molecules, organic acids and their derivatives, unclassified compounds, and phenylpropanes and polyketides.The correlation analysis showed that during testis development of Guanzhong dairy goats from 1 and 24 months old, lipids and lipid-like molecules had the largest positive correlation with glycerophospholipids, and carboxyl groups and their derivatives had the largest negative correlation with glycerophospholipids.The enrichment analysis of different metabolites resulted in 7 potentially important metabolic pathways, which were choline metabolism, arginine and proline metabolism, central carbon metabolism in tumor, ferroptosis, glutathione metabolism, aminoacyl-tRNA biocomposition, and taurine and hypotaurine metabolism.【Conclusion】 The results of this experiment revealed that the differential metabolites and metabolic mechanisms of testis in Guanzhong dairy goats at 1 and 24 months old from the perspective of metabolomics, and provided abasis for future research on the development of mammalian testis.

Key words: Guanzhong dairy goats; testis development; metabolomics; differential metabolites; metabolic pathways

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