混合油脂对清远麻鸡胸肌脂肪酸组成的影响及其代谢组机制研究

doi:10.16431/j.cnki.1671-7236.2020.04.011

Abstract

Abstract: This study was conducted to examine the effects of dietary addition of soybean oil or a blend of oil and fat on the growth performance and fatty acid profile of breast muscle in chickens,and to explore the mechanism of metabolic changes occurring in the fatty acid profile of breast muscle based on metabolomics technology,which could provide new ideas for regulating the fatty acid composition of chickens.A total of 480 one-day-old female chicks (Qingyuan) were randomly assigned into two dietary treatments,each consisted of six replicates and 40 chicks per replicate.The diet of control group (CON) was added with soybean oil,and the experimental group (BOF) was added with the blend of oil and fat,soybean oil∶lard∶fish oil∶coconut oil=1.0∶1.0∶0.5∶0.5.The experimental period was 127 d.The results showed that the birds fed the BOF diet,compared to the controls,had similar daily body weight gain (P > 0.05),lower daily feed intake and feed∶gain ratio (P < 0.05);higher content of lauric acid (C12∶0),myristic acid (C14∶0),oleic acid (C18∶1n-9),eicosapentaenoic acid (EPA,C20∶5n-3) and docosahexaenoic acid (DHA,C22∶6n-3) (P < 0.05),and lower content of linoleic acid (C18∶2n-6) (P < 0.05) in breast muscle tissue.A total of 110 different metabolites (VIP > 1,P < 0.05) in the breast muscle were detected by liquid chromatography-mass spectrometry(LC-MS),due to the dietary treatment,63 of which were annotated and were mainly phospholipid metabolites,including phosphatidylcholine (PC),phosphatidylserine (PS) and phosphatidylethanolamine (PE).In addition,the contents of glutathione and carnosine in breast muscle were significantly increased in the BOF treatment (P < 0.05).Through the metabolic pathway enrichment analysis,it was found that fatty acid metabolism pathways,including alpha-linolenic acid metabolism,arachidonic acid metabolism,linoleic acid metabolism and glycerophospholipid metabolism were changed significantly (P < 0.05).In summary,the BOF diet improved the feed efficiency of chickens and promoted DHA and EPA deposition in the breast muscle compared to those birds fed traditional soybean oil diet.It had been proven that the dietary fat intervention modified the phospholipid metabolites,oxidative stability metabolites and lipid metabolism pathways in breast muscle.The identified differential metabolites and differential metabolic pathways could provide references for the future studies on regulating fatty acid composition in chicken’s breast muscle.

Key words: Qingyuan chickens; blend of oil and fat; fatty acid composition; muscle metabolomics; phospholipid metabolites

CLC Number:

S816

GOU Zhongyong, CUI Xiaoyan, FAN Qiuli, LI Long, LIN Xiajing, WANG Yibing, JIANG Shouqun, JIANG Zongyong. Effects of Mixed Lipid on Breast Muscle Fatty Acid Composition, and Underlying Metabolomics Mechanism in Qingyuan Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1058-1069.

References

[1] RIBEIRO T,LORDELO M M,ALVES S P,et al.Direct supplementation of diet is the most efficient way of enriching broiler meat with n-3 long-chain polyunsaturated fatty acids[J].British Poultry Science,2013,54(6):753-765.
[2] CORTINAS L,VILLAVERDE C,GALOBART J,et al.Fatty acid content in chicken thigh and breast as affected by dietary polyunsaturation level[J].Poultry Science,2004,83(7):1155-1164.
[3] WANG J,WANG X,LI J,et al.Effects of dietary coconut oil as a medium-chain fatty acid source on performance,carcass composition and serum lipids in male broilers[J].Asian-Australasian Journal of Animal Sciences,2015,28(2):223-230.
[4] LÓPEZ-FERRER S,BAUCELLS M D,BARROETA A C,et al.n-3 enrichment of chicken meat.1.Use of very long-chain fatty acids in chicken diets and their influence on meat quality:Fish oil[J].Poultry Science,2001,80(6):741-752.
[5] 李娟娟.油脂类型和日粮能量及其互作对肉仔鸡脂肪代谢的影响[D].北京:中国农业科学院.2008. LI J J.Effects of oil types,dietary energy levels and interaction on fat metabolism of broilers[D].Beijing:Chinese Academy of Agricultural Sciences,2008.(in Chinese)
[6] RYMER C,GIBBS RGIVENS D.Comparison of algal and fish sources on the oxidative stability of poultry meat and its enrichment with omega-3 polyunsaturated fatty acids[J].Poultry Science,2010,89(1):150-159.
[7] 韩瑞丽,李同树,李建群,等.鱼油日粮对鸡肉品质的影响研究[J].中国家禽,2005,27(19):15-16. HAN R L,LI T S,LI J Q,et al.Study on the effect on chicken meat quality of fish oil diet[J].China Poultry,2005,27(19):15-16.(in Chinese)
[8] DAYRIT F M.The properties of lauric acid and their significance in coconut oil[J].Journal of the American Oil Chemists'Society,2015,92(1):1-15.
[9] ASSUNÇAO M L,FERREIRA H S,DOS SANTOS A F,et al.Effects of dietary coconut oil on the biochemical and anthropometric profiles of women presenting abdominal obesity[J].Lipids,2009,44(7):593-601.
[10] 禤小凤,邓福明,赵松林,等.椰子油的生理活性(Ⅱ):调节血浆血脂[J].热带农业科学,2013,33(9):65-70. XUAN X F,DENG F M,ZHAO S L,et al.Biological activity of coconut oil:Regulating the plasma lipid[J].Chinese Journal of Tropical Agriculture,2013,33(9):65-70.(in Chinese)
[11] 邓福明,王挥,赵松林,等.椰子油的生理活性(Ⅰ):药用功能[J].热带农业科学,2013,33(9):60-64. DENG F M,WANG H,ZHAO S L,et al.Biological activity of coconut oil:Medicinal functions[J].Chinese Journal of Tropical Agriculture,2013,33(9):60-64.(in Chinese)
[12] MARINA A M,MAN Y B,NAZIMAH S A,et al.Antioxidant capacity and phenolic acids of virgin coconut oil[J].International Journal of Food Sciences&Nutrition,2009,60(sup2):114-123.
[13] BHATNAGAR A,PRASANTH KUMAR P,HEMAVATHY J,et al.Fatty acid composition,oxidative stability,and radical scavenging activity of vegetable oil blends with coconut oil[J].Journal of the American Oil Chemists'Society,2009,86(10):991-999.
[14] 夏秋瑜,李瑞,唐敏敏,等.天然椰子油的组分及其对花生油氧化稳定性的影响[J].中国粮油学报,2012,27(9):64-66. XIA Q Y,LI R,TANG M M,et al.Components of natural coconut oil and its influences on oxidation stability of peanut oil[J].Journal of The Chinese Cereals and Oils Association,2012,27(9):64-66.(in Chinese)
[15] BAIRA E,DAGLA I,SIAPI E,et al.UHPLC-HRMS-based tissue untargeted metabolomics study of naringin and hesperidin after dietary supplementation in chickens[J].Food Chemistry,2018,269:276-285.
[16] PATTI G J,YANES O,SIUZDAK G.S.Innovation:Metabolomics:The apogee of the omics trilogy[J].Nature Reviews Molecular Cell Biology,2012,13(4):263-269.
[17] CUI X,LIU R,CUI H,et al.Effects of caponization and ovariectomy on objective indices related to meat quality in chickens[J].Poultry Science,2017,96(3):770-777.
[18] SACCENTI E,HOEFSLOOT H C,SMILDE A K,et al.Reflections on univariate and multivariate analysis of metabolomics data[J].Metabolomics,2014,10(3):361-374.
[19] WISHART D S,JEWISON T,GUO A C,et al.HMDB 3.0-the human metabolome database in 2013[J].Nucleic Acids Research,2012,41(D1):D801-D807.
[20] ZHU Z J,SCHULTZ A W,WANG J,et al.Liquid chromatography quadrupole time-of-flight mass spectrometry characterization of metabolites guided by the METLIN database[J].Nature Protocols,2013,8(3):451-460.
[21] ALPARSLAN G,ÖZDOGAN M.The effects of diet containing fish oil on some blood parameters and the performance values of broilers and cost efficiency[J].International Journal of Poultry Science,2006,5(5):415-419.
[22] 张亚男,齐博,张海军,等.不同油脂对肉仔鸡生长性能,屠宰性能和肉品质的影响[J].动物营养学报,2016,28(1):142-150. ZHANG Y N,QI B,ZHANG H J,et al.Effects of different oils on growth performance,carcass quality and meat quality in broilers[J].Chinese Journal of Animal Nutrition,2016,28(1):142-150.(in Chinese)
[23] NAVIDSHAD B.Effects of fish (Clupeonella cultriventris,Caspian sea originated) oil supplement on the serum lipoproteins and production of ω-3 fatty acids enriched broiler meat[J].Journal of Veterinary Research,2013,68(4):405-414.
[24] DURAN-MONTGÉ P,LIZARDO R,TORRALLARDONA D,et al.Fat and fatty acid digestibility of different fat sources in growing pigs[J].Livestock Science,2007,109(1):66-69.
[25] YAU J C,DENTON J H,BAILEY C A,et al.Customizing the fatty acid content of broiler tissues[J].Poultry Science,1991,70(1):167-172.
[26] KONIECZKA P,CZAUDERNA M,SMULIKOWSKA S.The enrichment of chicken meat with omega-3 fatty acids by dietary fish oil or its mixture with rapeseed or flaxseed-effect of feeding duration:Dietary fish oil,flaxseed,and rapeseed and n-3 enriched broiler meat[J].Animal Feed Science and Technology,2017,223:42-52.
[27] HARIHARA IYER M N,SARMAH B C,TAMULI M K,et al.Effect of dietary sunflower oil and coconut oil on adipose tissue gene expression,fatty acid composition and serum lipid profile of grower pigs[J].Archives of Animal Nutrition,2012,66(4):271-282.
[28] 聂存喜,张文举,闫理东,等.基于棉籽粕源发酵饲料的鸡血浆代谢组学研究[J].畜牧兽医学报,2013,44(5):737-744. NIE C X,ZHANG W J,YAN L D,et al.A metabolomics study on chicken plasma based on fermented fed from cotonsed meal mixed substrate[J].Acta Veterinaria et Zotechnica Sinica,2013,44(5):737-744.(in Chinese)
[29] 许梅燕.添加色素在鸡及其产品中代谢组学的GC-MS分析研究[D].长春:长春理工大学.2012. XU M Y.A GC-MS analytical study on the metabonomics of added pigments for the chickens and their products[D].Changchun:Changchun University of Science and Technology,2012.(in Chinese)
[30] 胥彩玉.饲粮中添加粪肠球菌对肉仔鸡生产性能,肉品质和血浆代谢组的影响[D].兰州:甘肃农业大学,2015. XU C Y.Effect of enterococcus faecalis on growth performance,meat quality and plasma metabolism in broiler chickens[D].Lanzhou:Gansu Agricultural University,2015.(in Chinese)
[31] GANDEMER G.Muscle lipid and meat quality.Phospholipids and flavor[J].Lipides,1997,4:19-25.
[32] 崔焕先.肉鸡肌内脂肪形成的分子调控网络及相关基因研究[D].北京:中国农业科学院.2011. CUI H X.Research on the molecular regulatory network and genes underlying intramuscular fat deposition in chickens[D].Beijing:Chinese Academy of Agricultural Sciences,2011.(in Chinese)
[33] XIAO Z,GE C,ZHOU G,et al.1H NMR-based metabolic characterization of Chinese Wuding chicken meat[J].Food Chemistry,2019,274:574-582.
[34] NEWMAN R E,BRYDEN W L,FLECK E,et al.Dietary n-3 and n-6 fatty acids alter avian metabolism:Molecular-species composition of breast-muscle phospholipids[J].British Journal of Nutrition,2002,88(1):19-28.
[35] DANNENBERGER D,NUERNBERG G,NUERNBERG K,et al.Effects of diets supplemented with n-3 or n-6 PUFA on pig muscle lipid metabolites measured by non-targeted LC-MS lipidomic profiling[J].Journal of Food Composition and Analysis,2017,56:47-54.
[36] NEVIN K,GRAJAMOHAN T.Wet and dry extraction of coconut oil:Impact on lipid metabolic and antioxidant status in cholesterol coadministered rats[J].Canadian Journal of Physiology and Pharmacology,2009,87(8):610-616.
[37] NEVIN K G,RAJAMOHAN T.Beneficial effects of virgin coconut oil on lipid parameters and in vitro LDL oxidation[J].Clinical Biochemistry,2004,37(9):830-835.
[38] 徐明生,欧阳克蕙,上官新晨,等.油脂对泰和乌骨鸡生产性能和肌肉脂肪酸组成的影响[J].福建农林大学学报(自然版),2006,35(1):67-72. XU M S,OUYANG K H,SHANGGUAN X C,et al.Effects of oils on the production performances and fatty acids composition of Taihe silky fowl[J].Journal of Fujian Agriculture and Forestry University (Natural Science Edition),2006,35(1):67-72.(in Chinese)
[39] CHASHNIDEL Y,MORAVEJ H,TOWHIDI A,et al.Influence of different levels of n-3 supplemented (fish oil) diet on performance,carcass quality and fat status in broilers[J].African Journal of Biotechnology,2010,9(5):687-691.
[40] FAMUREWA A C,AJA P M,MADUAGWUNA E K,et al.Antioxidant and anti-inflammatory effects of virgin coconut oil supplementation abrogate acute chemotherapy oxidative nephrotoxicity induced by anticancer drug methotrexate in rats[J].Biomedicine and Pharmacotherapy,2017,96:905-911.
[41] RAHIM N S,LIM S M,MANI V,et al.Enhanced memory in Wistar rats by virgin coconut oil is associated with increased antioxidative,cholinergic activities and reduced oxidative stress[J].Pharmaceutical Biology,2017,55(1):825-832.
[42] ANDREA G,ANDREA C,PAOLO R,et al.Carnosine and carnosine-related antioxidants:A review[J].Current Medicinal Chemistry,2005,12(20):2293-2315.
[43] 吕锦芳,宁康健,金光明,等.肌肽对肉鸡抗氧化功能影响的研究[J].中国饲料,2007,15:21-23. LYU J F,NING K J,JIN G M,et al.Effect of carnosine on antioxidant function of broilers[J].China Feed,2007,15:21-23.(in Chinese)

Effects of Mixed Lipid on Breast Muscle Fatty Acid Composition, and Underlying Metabolomics Mechanism in Qingyuan Chickens

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 3

Recommended Articles

Metrics

Comments

[1]	FENG Jia, ZHANG Haijun, WU Shugeng, MIN Yuna, QI Guanghai, WANG Jing, GAO Yupeng. Effect of Dietary Flaxseed Oil Supplementation on Yolk Fatty Acid Composition and Sensory Profile of Eggs [J]. , 2018, 45(10): 2733-2742.
[2]	QIU Ju, LIU Peng, LIANG Ke-hong, HAN Juan, QIN Yu-chang. Effect of Different Domestic Alfalfa Adding Content on Nutritional Quality of Local Fresh Milk [J]. , 2017, 44(5): 1393-1398.
[3]	ZHANG Ting, ZHONG Wei, LUO Jing, XU Chao, HUANG Jian, ZHOU Ning, LI Guang-yu. Effect of Dietary Fatty Acid Composition on Growth Performance,Nutrient Digestion and Nitrogen Metabolism of Silver Fox in the Growing Period [J]. , 2014, 41(12): 141-145.