黔北麻羊MC4R基因多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2023.01.020

Abstract

Abstract: 【Objective】 The aim of this study was to explore melanocortin receptor-4 (MC4R) gene polymorphism and its correlation with growth traits in Qianbei Ma goats, in order to provide theoretical basis for breed selection and breeding of Qianbei Ma goats.【Method】 196 six-month-old Qianbei Ma goats were used as experimental materials, the single nucleotide polymorphism (SNP) of MC4R gene was screened by DNA mixing pool combined with Sanger sequencing, and the general linear model (GLM) in SPSS 22.0 software was used to analyze the correlation between SNP of MC4R gene and growth traits of Qianbei Ma goats.【Result】 There were 4 SNPs of MC4R gene in Qianbei Ma goats, g.59345469 C>A and g.59346773 T>C were located in the 5'- and 3'-untranslated region (UTR), respectively.The mutation of g.59345871 A>C resulted in the transformation of aspartic acid (Asp) to alanine (Ala) at position 37, and caused significant changes in the secondary structure of RNA and the secondary and tertiary structures of protein.The mutation of g.59346263 A>G resulted in the transformation of isoleucine (Ile) to valine (Val) at position 168, which had significant effects on the secondary structure of RNA and the secondary structure of protein.The results of association analysis showed that g.59345469 C>A had significant effect on the body weight, chest circumference and cannon circumference in Qianbei Ma goats (P<0.05). g.59345871 A>C had significant effect on the body weight, body height, chest circumference and cannon circumference in Qianbei Ma goats (P<0.05).g.59346263 A>G had significant effects on the body height, body weight and chest circumference in Qianbei Ma goats (P<0.05).g.59346773 T>C had significant effect on the body weight and chest circumference in Qianbei Ma goats (P<0.05).A total of 9 haplotypes and 21 diplotypes were detected by the combination of 4 SNPs, the combination had significant genetic effects on the body weight, body height, chest circumference and cannon circumference in Qianbei Ma goats (P<0.05), the growth traits of homozygous diplotype H5H5 (AAAAGGCC) individual was superior to that of other diplotypes.【Conclusion】 Four SNPs of MC4R gene were significantly associated with the body weight and chest circumference in Qianbei Ma goats, which could be used as genetic markers for molecular breeding of Qianbei Ma goats.

Key words: Qianbei Ma goats; MC4R gene; single nucleotide polymorphism (SNP); growth traits

CLC Number:

S813.3

LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.

References

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Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats

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