延边黄牛lncRNA MEG3基因多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.12.025

Abstract

Abstract: The aim of this study was to investigate the association between long noncoding RNA (lncRNA) maternally expressed gene 3 (MEG3) gene single nucleotide polymorphism (SNP) and the growth traits in Yanbian Yellow cattle.DNA direct sequencing and high resolution melting curve (HRM) were used to analyze the polymorphism of MEG3 gene in 90 Yanbian Yellow cattle at 24 months of age, and association analysis was performed combined with 13 growth traits such as chest width, thurl width and perimeter of abdomen.The results showed that MEG3 gene exon 5 contained two mutations of g.65745819 G>A and g.65745850 C>A, both of them had two alleles (G, A and C, A), the allele frequency were 0.661, 0.339 and 0.778, 0.222, respectively, and produced three genotypes(GG, GA, AA and CC, CA and AA), the genotype frequency were 0.422, 0.478, 0.100 and 0.611, 0.333, 0.056, respectively.χ² test showed no deviation from Hardy-Weinberg equilibrium (P>0.05).The genetic homozygosity, heterozygosity, effective number of alleles and polymorphism information content (PIC) of the two loci were 0.552, 0.448, 1.812, 0.348 and 0.286, 0.346, 1.528, 0.286, respectively, which were in moderate polymorphism (0.25<PIC<0.5).Association analysis showed that the chest width of GA genotype at g.65745819 G>A was significantly higher than that of AA genotype (P<0.05), and the thurl width of AA genotype was significantly higher than that of GG and GA genotypes (P<0.05).The perimeter of abdomen trait of AA genotype at g.65745850 C>A was significantly higher than that of CA genotype (P<0.05), but there was no significant difference among other genotypes and traits (P>0.05).The results indicated that the polymorphism of lncRNA MEG3 gene was associated with some growth traits of Yanbian Yellow cattle, and could be used as a candidate gene for molecular marker assisted breeding of Yanbian Yellow cattle.

Key words: Yanbian Yellow cattle; lncRNA; MEG3 gene; SNP; growth traits

CLC Number:

S813.3

TIAN Quan, ZHAO Yi, WANG Zhuo, LIU Lei, ZHANG Junzheng, JI Shuang. Polymorphisms of lncRNA MEG3 Gene and Its Correlation with Growth Traits in Yanbian Yellow Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4567-4574.

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Polymorphisms of lncRNA MEG3 Gene and Its Correlation with Growth Traits in Yanbian Yellow Cattle

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