野生盘羊×巴什拜羊杂交二代尾部脂肪差异表达lncRNA的鉴定与分析

doi:10.16431/j.cnki.1671-7236.2022.05.020

Abstract

Abstract: 【Objective】 The purpose of this study was to analyze the expression profile of long non-coding RNA (lncRNA) in tail fat tissues of different tail fat-type sheep,and explore the relationship between lncRNA and tail fat deposition mechanism in sheep,so as to provide a theoretical basis for revealing the mechanism of tail fat deposition in sheep.【Method】 Xinjiang local sheep Bashibai sheep(fat tail-type) and the hybrid second generation of wild Argali×Bashibai sheep (small tail-type) were selected as the research objects.The tail fat tissues of two populations were collected,the transcriptome sequencing technology was used to screen the differentially expressed lncRNA and predict target gene,and GO function and KEGG pathway enrichment were analyzed.The expression results of transcriptome sequencing were verified by Real-time quantitative PCR.【Result】 728 differentially expressed lncRNAs were screened by differential expression analysis,of which 270 lncRNAs were down-regulated,and the expression of 458 lncRNAs was up-regulated.The target gene was screened by GO function and KEGG pathway enrichment analysis of differentially expressed lncRNA,there were 634 target genes involved in diseases,immunity,protein modification,cell metabolism and other related functions,involving a total of 76 signal pathways.Some lncRNA-mediated target genes SCD,GPAM,THRSP,FASN,etc,were related to tail fat deposition in sheep.The result of Real-time quantitative PCR and transcriptome sequencing was consistent.【Conclusion】 lncRNA played an important role in regulating tail fat deposition in the evolution of tail fat in sheep,which provided theoretical basis for analyzing fat deposition in sheep from the perspective of lncRNA.

Key words: sheep; tail fat; fat deposition; lncRNA; RNA-Seq

CLC Number:

S826

SU Xiaohui, HE Haiying, TUERXUNAYI·Yalikun, WANG Qiong, MA Haiyu, LIU Lingling, LIU Wujun. Identification and Analysis of Differentially Expressed lncRNA of Tail Fat in the Second Generation of Crossbred from Wild Argali×Bashibai Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1796-1805.

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Identification and Analysis of Differentially Expressed lncRNA of Tail Fat in the Second Generation of Crossbred from Wild Argali×Bashibai Sheep

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