中国荷斯坦牛PIK3CB基因多态性及其与繁殖和产奶性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.09.018

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the relationships between the phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit beta (PIK3CB) gene polymorphism and reproductive and milk production traits in Chinese Holstein cattle.【Method】 The single nucleotide polymorphisms (SNP) site of PIK3CB gene in Chinese Holstein cattle was screened using hybrid pool sequencing.SNP genotyping and population genetic analysis were performed in 1 160 Holstein dairy cows based on Kompetitive Allele Specific PCR (KASP) method.The linear model was employed to perform the association between SNP and 11 reproductive and milk production traits based on unit point and haplotype combinations.【Results】 A total of 17 SNPs were detected in PIK3CB gene, and 7 SNPs were screened for subsequent analysis.The association analysis revealed that a total of 7 SNPs were significantly or extremely significantly associated with various target traits (P<0.05 or P<0.01).The individuals with AA genotype at g.130433743 A>G in exon and GG genotype at g.130448069 G>A in variable splice region had the lowest mating interval, milk yield, milk protein and milk fat, and the highest somatic cell score, which suggested the individuals with the above genotypes had shorter mating intervals between the first and last mating and relatively poor milk production performance.The individuals with AA genotype at g.130387717 G>A had the lowest mating age and the lowest first to last mating interval of young cattle, and the highest milk yield, milk protein yield and milk fat.The above mentioned 3 SNPs could be considered as candidate SNPs for reproductive and milk production traits in Chinese Holstein cattle.Haplotype analysis revealed that 6 SNPs of g.130387717 G>A, g.130430832 A>-, g.130433743 A>G, g.130433982 C>T, g.130446073 C>T and g.130448069 G>A were closely linked to form a haplotype block, and were significantly or extremely significantly associated with various target traits (P<0.05 or P<0.01).Among the various haplotypes, H2H3 and H2H4 haplotype combinations had better reproductive and milk-producing performance, and were the advantageous haplotype combination.【Conclusion】 Polymorphism of PIK3CB gene had abundant variants which was associated with reproductive and milk production traits in Chinese Holstein cattle. g.130433743 A>G, g.130448069 G>A and g.130387717 G>A could be considered as potential molecular markers to provide the theoretical basis for balanced breeding of Chinese Holstein cattle.

Key words: Chinese Holstein cattle; PIK3CB gene; single nucleotide polymorphism (SNP); reproductive trait; milk production trait

CLC Number:

S823

XU Haoqi, XU Jingyi, HU Lirong, ZHANG Fan, LUO Hanpeng, ZHANG Hailiang, SHI Rui, LI Xiang, LIU Lin, LIU Qiaoxiang, GUO Gang, WANG Yachun. Polymorphism of PIK3CB Gene and Its Association Analysis with Reproductive and Milk Production Traits in Chinese Holstein Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3438-3452.

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Polymorphism of PIK3CB Gene and Its Association Analysis with Reproductive and Milk Production Traits in Chinese Holstein Cattle

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