China Animal Husbandry and Veterinary Medicine ›› 2022, Vol. 49 ›› Issue (6): 2176-2185.doi: 10.16431/j.cnki.1671-7236.2022.06.018

• Genetics and Breeding • Previous Articles     Next Articles

Screening of lncRNA Related to Inbreeding Depression of Reproduction in Chickens

XUE Qian1,2, LI Guohui1,2, XING Weijie1,2, ZHOU Chenghao1,2, ZHANG Huiyong1,2, YIN Jianmei1,2, ZHU Yunfen1,2, CAO Yuxia1,2, SU Yijun1,2, HAN Wei1,2   

  1. 1. Science and Technology Innovation Co., Ltd., Poultry Institute of Jiangsu Province, Yangzhou 225125, China;
    2. Poultry Institute of Jiangsu Province, Yangzhou 225125, China
  • Received:2021-11-03 Online:2022-06-05 Published:2022-05-27

Abstract: 【Objective】 This study was aimed to screen long non-coding RNA (lncRNA) related to inbreeding depression of reproduction in chickens, so as to provide reference for further exploring the regulation mechanism of lncRNA on inbreeding depression of reproduction in chickens.【Method】 The strongly and weakly inbreeding populations of Langshan chickens were used as materials, lncRNA expression in hypothalamus and ovary of Langshan chickens was analyzed by transcriptome sequencing.Differentially expressed lncRNA between strongly and weakly inbreeding populations were identified, and cis regulation target gene prediction and function analysis were performed.【Result】 A total of 4 222 lncRNAs were identified in hypothalamus and ovary of Langshan chickens.Comparing the strongly and weakly inbreeding populations, 35 and 215 differentially expressed lncRNAs were found in hypothalamus and ovary, respectively.In hypothalamus, 98 cis regulation target genes were predicted in differentially expressed lncRNAs, which were significantly enriched in reproduction-related biological processes (P<0.05), such as embryo development ending in birth or egg hatching, embryonic heart tube development and response to retinoic acid, involving MSTRG.9196.4, MSTRG.9195.2, MSTRG.6254.2 lncRNAs and their corresponding target genes DNAAF2 and FKBP1B.In ovary, 414 target genes were predicted, which were enriched in oocyte meiosis, MAPK and folic acid synthesis signaling pathways, including MSTRG.7683.1, MSTRG.13604.4, MSTRG.16570.1, MSTRG.8330.5, MSTRG.8330.4 and other lncRNAs related to neuroendocrine regulation and gametogenesis.【Conclusion】 In this study, a series of differential lncRNAs related to embryo development and gametogenesis in chickens were screened from hypothalamus and ovary.These lncRNAs could be used as candidate lncRNAs for inbreeding depression of reproduction in chickens, and provided clues for revealing the regulation mechanism of inbreeding depression in reproduction of chickens.

Key words: chickens; reproductive traits; inbreeding depression; differential expression; long non-coding RNA (lncRNA); target gene

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