bta-miR-34b/c和bta-miR-449a/b/c靶基因预测及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2022.07.003

Abstract

Abstract: 【Objective】 This study was aimed to predict and analyze the sequence conservation,transcription factors and target genes of bta-miR-34b/c and bta-miR-449a/b/c by bioinformatics,and explore the mechanism which affected spermatogenesis,so as to provide guidance and ideas for studying its biological function and mechanism of action.【Method】 The miRBase database was used to obtain and analyze the sequence characteristics of bta-miR-34b/c and bta-miR-449a/b/c in different species for similarity.TargetScan,miRWalk,miRDB and other methods were used to predict target genes of bta-miR-34b/c and bta-miR-449a/b/c,and the target genes were performed to GO functional and KEGG pathway enrichment analysis.The transcription factors that simultaneously regulated bta-miR-34b/c and bta-miR-449a/b/c were obtained by AnimalTFDB,and the TF-miRNA-mRNA interaction network was constructed.【Result】 bta-miR-34b/c and bta-miR-449a/b/c were highly conserved among different species.According to the prediction results,2 transcription factors and 49 candidate target genes were obtained.These target genes were mainly enriched in spermatogenesis,regulation of calcium-dependent exocytosis,regulation of insulin secretion and other GO items,as well as HIF-1 signaling pathway and thyroid hormone signaling pathway,Wnt signaling pathway and other KEGG pathways.The interaction network composed of bta-miR-34b/c,bta-miR-449a/b/c,upstream transcription factors NR2C2 and HIC1,and downstream target genes AXL,E2F3,DAAM1,etc affected spermatogenesis by regulating meiosis,cell cycle,apoptosis,sperm cell energy metabolism and other biological processes.【Conclusion】 The spermatogenesis were regulated by molecular interactive network of bta-miR-34b/c and bta-miR-449a/b/c with transcription factors and target genes.

Key words: miR-34b/c; miR-449a/b/c; spermatogenesis; target genes; transcription factors

CLC Number:

DU Xinze, MA Xinhao, ZHANG Dianqi, DU Jiawei, MA Jing, XIE Kuncheng, HE Jie, ZAN Linsen. Target Gene Prediction and Bioinformatics Analysis of bta-miR-34b/c and bta-miR-449a/b/c[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2451-2461.

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Target Gene Prediction and Bioinformatics Analysis of bta-miR-34b/c and bta-miR-449a/b/c

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