绵羊睾丸差异基因及蛋白质互作网络关系研究

doi:10.16431/j.cnki.1671-7236.2022.01.001

Abstract

Abstract: [Objective] The research was aimed to integrate and analyze the multiple transcription data sets of testis in sheep, reveal the differential genes and protein interaction network of testis in sheep, and explore the key genes affecting spermatogenesis in sheep, so as to provide theoretical reference for reproduction in sheep. [Method] 74 testis transcriptome in sheep were bioinformatic analyzed, differential genes were carried out by limma package, WGCNA was used to construct the weighted differential genes co-expression network of testis in sheep and MCODE was used to calculate the important genes in the network. Metascape was used for functional enrichment analysis, and Cytoscape plug-in AutoAnnotate was used to identify gene clusters. [Results] 11 884 genes were screened and 237 366 protein interactions were constructed. Protein interaction network were constructed from 2 058 differential genes, and 46 169 protein interaction relationship were generated. 4 gene sets with the highest scores were identified. At the same time, a weighted co-expression network was constructed for 2 058 differential genes, and a total of 7 modules were obtained, among which 929 genes in the Blue module were found to be significantly enriched in male gamete production, reproduction, spermatogenesis, flagellum motility and AMPK signaling pathway, and the enrichment results were highly connected and aggregated into a complete network. As a result, 25 key genes involved in spermatogenesis and sperm cell development. [Conclusion] This study revealed the protein interaction network of genes expressed in testis of sheep and the protein interaction network of multi-dimensional differential genes, finally 25 genes were associated with testis spermatogenesis and interaction relationship, it provided theoretical basis for breeding in sheep.

Key words: sheep; testis differential gene; protein interaction network; key gene set; spermatogenesis

CLC Number:

S814.8

LIU Zaixia, DUAN Shi, SUN Yanyong, LYU Qi, FU Shaoyin, HE Xiaolong, ZHANG Wenguang, LIU Yongbin. Study on Differential Gene and Protein Interaction Network of Testis in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 1-11.

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Study on Differential Gene and Protein Interaction Network of Testis in Sheep

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