miR-383在牦牛、犏牛睾丸组织中的差异表达及功能预测

doi:10.16431/j.cnki.1671-7236.2016.02.005

Abstract

Abstract: The aim of this study was to detect the differential expression of miR-383 in yak and cattle-yak testis,and explore the important roles of miR-383 in spermatogenesis.Using the testis tissue of mature yak and cattle-yak as experimental material,Real-time PCR was performed to detect the miR-383 expression.miR-383 targets were obtained using TargetScan and miRanda database,and biological functions of these target genes were predicted by David and Gene Ontology online softwares.The miR-383 had significantly differential expression in F₁ testis tissue between yak and cattle-yak(P<0.05),and it had the highest expression level in yak testis tissues.131 target genes were obtained by target gene prediction which involved in cell proliferation,apoptosis,regulation of nucleic acid synthesis and other processes by GO analysis and KEGG pathway analysis.The results showed that miR-383 might participate in the regulation of germ cell differentiation and spermatogenesis,which would provide clues and theoretical basis for studying the function and regulation mechanism of miR-383 in cattle-yak germ cell.

Key words: yak; cattle-yak; miR-383; spermatogenesis; male sterility; bioinformatics analysis

CLC Number:

Q786

LIAO Ke, CHAI Zhi-xin, ZHANG Si-yuan, ZHONG Jin-cheng. Differential Expression and Function Prediction of miR-383 in Yak and Cattle-yak Testis[J]. , 2016, 43(2): 319-325.

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Differential Expression and Function Prediction of miR-383 in Yak and Cattle-yak Testis

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