鸡Wnt基因家族生物信息学分析及表达

doi:10.16431/j.cnki.1671-7236.2023.01.002

Abstract

Abstract: 【Objective】 This study was aimed to conduct a comprehensive and systematic investigation and analysis of Wnt gene family in chickens, and further clarify the expression patterns of Wnt gene family members in chickens during Newcastle disease virus (NDV) infection.【Method】 Based on chicken genome sequencing results, the whole genome sequence of Wnt gene family in chickens was obtained by querying the Pfam and UniPort databases, the phylogenetic tree was constructed, and its physicochemical properties, subcellular protein localization, chromosome distribution, conservative domain, and so on.The expression pattern of Wnt gene family members in chickens infected with NDV was analyzed.【Result】 Thirty-nine Wnt gene family members were identified.The phylogenetic tree analysis showed that there was a high similarity of Wnt members among chicken, human and mouse, there were 12 subfamilies, namely Wnt1, Wnt2(Wnt2a and Wnt2b), Wnt3a, Wnt4, Wnt5(Wnt5a and Wnt5b), Wnt6, Wnt7(Wnt7a and Wnt7b), Wnt8(Wnt8a and Wnt8b), Wnt9(Wnt9a and Wnt9b), Wnt10a, Wnt11 and Wnt16. However, the chicken lacked Wnt10b.Wnt members in chickens distributed on 11 chromosomes (Chr1, Chr2, Chr4, Chr6, Chr7, Chr12, Chr13, Chr21, Chr26, Chr27 and CHR34, respectively).There were 14 tandem repeats among the members, and no segment repeat occurred.There was 10 conservative motifs in 39 Wnt members of chickens, the 3'-end of Wnt gene family members were relatively conservative, except for rna-XM_040653060.2(Wnt6) and rna-XM_040650634.2(Wnt5a), the 3'-end of the other Wnt members were composed of motifs 2 and 5.In addition, 39 Wnt family members of chickens all contained conservative regions, namely motifs 1 and 3. Analysis of transcriptome data revealed that the expression of Wnt7a was significantly up-regulated during NDV infection.【Conclusion】 The Wnt gene family members in chickens presented non-uniform distribution on the chromosome, and the gene structure was relatively conservative.Tandem repeat was the primary amplification mode of Wnt gene family members.Wnt7a might be closely related to Wnt regulating macrophages to participate in microbial infection.

Key words: chickens; Wnt gene family; bioinformatics; expression

CLC Number:

S831

LI Xinran, ZHANG Qingyan, ZHA Weiwei, ZHU Miao. Bioinformatics Analysis and Expression of Wnt Gene Family in Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 15-25.

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Bioinformatics Analysis and Expression of Wnt Gene Family in Chickens

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