黔东南小香鸡MEF2C基因外显子多态性及其与体重、体尺性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.07.019

Abstract

Abstract: 【Objective】 This study was aimed to investigate the effect of myocyte enhancer factor 2C (MEF2C) gene polymorphism on the body weight and body size traits in Qiandongnan Xiaoxiang chickens.【Method】 251 300-day-old Qiandongnan Xiaoxiang chickens were used as the research object,the polymorphism of all (15) exons of MEF2C gene were analyzed by PCR amplification and direct sequencing technology,and the single nucleotide polymorphism (SNP) was screened.The correlation between the genotypes corresponding to different SNPs and body weight or body size traits in Qiandongnan Xiaoxiang chickens were analyzed using SPSS 18.0 statistical analysis software.【Result】 Only 3 SNPs (g.1819 T＞C,g.2426 T＞C and g.2543 C＞T) were found in exon 12 of MEF2C gene in Qiandongnan Xiaoxiang chickens,and no SNP was found in other exons.χ² test results showed that 3 SNPs deviated from Hardy-Weinberg equilibrium in Qiandongnan Xiaoxiang chickens (P＞0.05).The results of association analysis showed that the body oblique length,chest depth,and tibia circumference of g.1819 T＞C TC genotype were extremely significantly higher than those of TT and CC genotypes,and the body weight was extremely significantly higher than that of TT genotype (P＜0.01);The tibial length of TC genotype was significantly higher than that of TT and CC genotypes,and the keel length was significantly higher than that of TT genotype (P＜0.05).g.2426 T＞C and g.2543 C＞T were linkage balance sites,which had extremely significant effect on the tibia circumference (P＜0.01).【Conclusion】 The exon of MEF2C gene in Qiandongnan Xiaoxiang chickens were highly conserved,and g.1819 T＞C could be used as a reference site for molecular marker-assisted breeding of Qiandongnan Xiaoxiang chickens.

Key words: Qiandongnan Xiaoxiang chickens; MEF2C gene; polymorphism; body weight; body size; association analysis

CLC Number:

S831.2

ZHOU Di, AO Ye, JIANG Guirong, ZHAO Zhonghai, LI Jun, YANG Rong, GONG Fei, CHEN Changxue, SHEN Yin, LI Hui. Polymorphism of MEF2C Gene Exon and Its Association Analysis with Weight and Body Size Traits in Qiandongnan Xiaoxiang Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2613-2621.

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Polymorphism of MEF2C Gene Exon and Its Association Analysis with Weight and Body Size Traits in Qiandongnan Xiaoxiang Chickens

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