China Animal Husbandry and Veterinary Medicine ›› 2022, Vol. 49 ›› Issue (7): 2613-2621.doi: 10.16431/j.cnki.1671-7236.2022.07.019

• Genetics and Breeding • Previous Articles     Next Articles

Polymorphism of MEF2C Gene Exon and Its Association Analysis with Weight and Body Size Traits in Qiandongnan Xiaoxiang Chickens

ZHOU Di1, AO Ye1, JIANG Guirong1, ZHAO Zhonghai3, LI Jun1, YANG Rong1, GONG Fei1, CHEN Changxue1, SHEN Yin1, LI Hui2   

  1. 1. Testing Center for Livestock and Poultry Germplasm, Guiyang 550018, China;
    2. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, China;
    3. Animal Husbandry and Fishery Industry Office of Zunyi City,Zunyi 563000, China
  • Received:2022-02-11 Online:2022-07-05 Published:2022-06-29

Abstract: 【Objective】 This study was aimed to investigate the effect of myocyte enhancer factor 2C (MEF2C) gene polymorphism on the body weight and body size traits in Qiandongnan Xiaoxiang chickens.【Method】 251 300-day-old Qiandongnan Xiaoxiang chickens were used as the research object,the polymorphism of all (15) exons of MEF2C gene were analyzed by PCR amplification and direct sequencing technology,and the single nucleotide polymorphism (SNP) was screened.The correlation between the genotypes corresponding to different SNPs and body weight or body size traits in Qiandongnan Xiaoxiang chickens were analyzed using SPSS 18.0 statistical analysis software.【Result】 Only 3 SNPs (g.1819 T>C,g.2426 T>C and g.2543 C>T) were found in exon 12 of MEF2C gene in Qiandongnan Xiaoxiang chickens,and no SNP was found in other exons.χ2 test results showed that 3 SNPs deviated from Hardy-Weinberg equilibrium in Qiandongnan Xiaoxiang chickens (P>0.05).The results of association analysis showed that the body oblique length,chest depth,and tibia circumference of g.1819 T>C TC genotype were extremely significantly higher than those of TT and CC genotypes,and the body weight was extremely significantly higher than that of TT genotype (P<0.01);The tibial length of TC genotype was significantly higher than that of TT and CC genotypes,and the keel length was significantly higher than that of TT genotype (P<0.05).g.2426 T>C and g.2543 C>T were linkage balance sites,which had extremely significant effect on the tibia circumference (P<0.01).【Conclusion】 The exon of MEF2C gene in Qiandongnan Xiaoxiang chickens were highly conserved,and g.1819 T>C could be used as a reference site for molecular marker-assisted breeding of Qiandongnan Xiaoxiang chickens.

Key words: Qiandongnan Xiaoxiang chickens; MEF2C gene; polymorphism; body weight; body size; association analysis

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