中国草原红牛PDK4基因多态性及其与肉质性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.06.019

Abstract

Abstract: 【Objective】 The aim of the expriment was to study on the relationship between PDK4 gene polymorphism and meat quality traits in Chinese Steppe Red cattle.【Method】 120 Chinese Steppe Red cattle were selected as the research object.The single nucleotide polymorphism (SNP) of exons 1-11 of PDK4 gene was detected by Sanger sequencing, and the genotype frequency, gene frequency and population genetic parameters of SNP were analyzed.SPSS 21.0 software was used to analyze the association between SNP polymorphism of PDK4 gene and meat quality traits (cooked meat rate, meat tenderness, water loss rate, pH, drip loss, intramuscular fat content, initial water content and protein content) in Chinese Steppe Red cattle.【Result】 Three SNPs were detected in exons 8 and 11 of PDK4 gene in Chinese Steppe Red cattle.There was a SNP site at 57 bp (G57C) in exon 8 of PDK4 gene, which caused the change of coding amino acids.There were GG, GC and CC genotypes at G57C site.There were two SNPs at 330 bp(G330T) and 389 bp(C389T) in exon 11 of PDK4 gene.There were three genotypes of GG, GT and TT at G330T and three genotypes CC, CT and TT at C389T.Chi-square test results showed that the G57C site of exon 8 of Chinese Steppe Red cattle deviated from Hardy-Weinberg equilibrium (P<0.05), and the G330T and C398T of exon 11 conformed to Hardy-Weinberg equilibrium (P>0.05).The analysis of population genetic parameters showed that the G57C site in exon 8 belonged to low degree polymorphism (PIC<0.25), and the number of alleles was 1.1429, indicating that it had little variation in Chinese Steppe Red cattle.Exon 11 G330T and C398T belong to moderate polymorphism sites (0.25<PIC<0.5), and the number of alleles were 1.6431 and 1.6447 respectively, indicating that the genetic marker could provide genetic information.The association analysis results showed that the intramuscular fat content of GG and CC genotypes at G57C site in exon 8 of PDK4 gene was significantly higher than that of GC genotype (P<0.05).The drip loss and initial water component of GG genotype at G330T in exon 11 were significantly higher than those of GT genotype (P<0.05).The meat tenderness of GG genotype was significantly higher than that of TT genotype (P<0.05).The intramuscular fat content of GT genotype was significantly higher than that of TT genotype (P<0.05), and the dehydration rate of CT genotype at C389T was significantly lower than that of TT genotype (P<0.05).【Conclusion】 PDK4 gene polymorphism was associated with meat quality traits in Chinese Steppe Red cattle, and could be used as a candidate gene for meat quality traits.

Key words: PDK4 gene; Chinese Steppe Red cattle; meat quality traits; polymorphism; association analysis

CLC Number:

S813.3

MA Yanru, YU Yongsheng, CAO Yang, QIN Lihong, ZHAO Yumin, WU Jian. Polymorphism of PDK4 Gene and Its Association with Meat Quality Traits in Chinese Steppe Red Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2186-2194.

References

[1] SCOLLAN N D, PRICE E M, MORGAN S A, et al.Can we improve the nutritional quality of meat?[J].Proceedings of the Nutrition Society, 2017, 76(4):603-618.
[2] SORET B, MENDIZABAL J A, ARANA A, et al.Expression of genes involved in adipogenesis and lipid metabolism in subcutaneous adipose tissue and longissimus muscle in low-marbled Pirenaica beef cattle[J].Animal, 2016, 10(12):2018-2026
[3] PARK S J, BEAK S H, JUNG D J S, et al.Genetic, management, and nutritional factors affecting intramuscular fat deposition in beef cattle-A review[J].Asian-Australasian Journal of Animal Sciences, 2018, 31(7):1043-1061.
[4] 吴健, 张国梁, 刘基伟, 等.吉林省中国草原红牛培育及选育提高进程[J].中国畜牧兽医, 2008, 35(11):152-155. WU J, ZHANG G L, LIU J W, et al.Jilin province, China Grassland Red cattle breeding and improvement process[J].China Animal Husbandry & Veterinary Medicine, 2008, 35(11):152-155.(in Chinese)
[5] 吴长庆, 于洪春, 张国良, 等.中国草原红牛品种资源现状及展望[J].黄牛杂志, 2000, 6:44-46. WU C Q, YU H C, ZHANG G L, et al.Status and prospect of Grassland Red cattle breed resources in China[J].Journal of Yellow Cattle Science, 2000, 6:44-46.(in Chinese)
[6] SUGDEN M C, HOLNESS M J.Therapeutic potential of the mammalian pyruvate dehydrogenase kinases in the prevention of hyperglycaemia[J].Current Drug Targets.Immune Endocrine and Metabolic Disorders, 2002, 2(2):151-165.
[7] BEHAL R H, BUXTON D B, ROBERTSON J G, et al.Regulation of the pyruvate dehydrogenase multienzyme complex[J].Annual Review of Nutrition, 1993, 13:497-520.
[8] ROWLES J, SCHERER S W, XI T, et al.Cloning and characterization of PDK4 on 7q21.3 encoding a fourth pyruvate dehydrogenase kinase isoenzyme in human[J].Journal of Biological Chemistry, 1996, 13;271(37):22376-22382.
[9] WHITE U A, COULTER A A, MILES T K, et al.The STAT5A-mediated induction of pyruvate dehydrogenase kinase 4 expression by prolactin or growth hormone in adipocytes[J].Diabetes, 2007, 56(6):1623-1629.
[10] 曹行, 何美升, 于茜, 等.伊犁马PDK4基因多态性与耐力性能的相关性研究[J].新疆农业大学学报, 2016, 39(4):302-306. CAO H, HE M S, YU X, et al.Polymorphism of PDK4 gene in Yili horse and endurance performance[J].Journal of Xinjiang Agricultural University, 2016, 39(4):302-306.(in Chinese)
[11] 赵伟明, 吴慧光, 吴江鸿, 等.基于转录组测序分析西门塔尔牛和安格斯牛脂肪沉积相关基因的差异表达[J].农业生物技术学报, 2020, 28(4):693-701. ZHAO W M, WU H G, WU J H, et al.Analysis of differentially expressed genes related to fat deposition in simmental and Angus cattle(Bos taurus) based on transcriptome sequencing[J].Journal of Agricultural Biotechnology, 2020, 28(4):693-701.(in Chinese)
[12] 中华人民共和国农业部.肉牛生产性能测定技术规范:NY/T 2660-2014[S].北京:中国标准出版社, 2014. MINISTRY OF AGRICULTURE, PRC.Technical specification for performance measurement of beef cattle:NY/T 2660-2014[S].Beijing:Standards Press of China, 2014(in Chinese)
[13] GUDA M R, ASUTHKAR S, LABAK C M, et al.Targeting PDK4 inhibits breast cancer metabolism[J].American Journal of Cancer Research, 2018, 8(9):1725-1738.
[14] PARK B Y, JEON J H, GO Y, et al.PDK4 deficiency suppresses hepatic glucagon signaling by decreasing cAMP levels[J].Diabetes, 2018, 67(10):2054-2068.
[15] ZHAO Y, TRAN M, WANG L, et al.PDK4-deficiency reprograms intrahepatic glucose and lipid metabolism to facilitate liver regeneration in mice[J].Hepatology Communication, 2020, 4(4):504-517.
[16] WANG J, QIAN Y, GAO M.Overexpression of PDK4 is associated with cell proliferation, drug resistance and poor prognosis in ovarian cancer[J].Cancer Management and Research, 2018, 11:251-262.
[17] WU C, ZHANG H, LIN X, et al.Role of PDK4 in insulin signaling pathway in periadrenal adipose tissue of pheochromocytoma patients[J].Endocrine Related Cancer, 2020, 27(10):583-589.
[18] LECLERC D, PHAM D N, LÉVESQUE N, et al.Oncogenic role of PDK4 in human colon cancer cells[J].British Journal of Cancer, 2017, 116(7):930-936.
[19] 杨丽华, 白东义, 高雅罕, 等.蒙古马运动相关基因的表达量差异研究[J].畜牧兽医学报, 2013, 44(12):1900-1906. YANG L H, BAI D Y, GAO Y H, et al.Characterization of exercise relevant genes in Mongolian horses[J].Acta Veterinaria et Zootechnica Sinica, 2013, 44(12):1900-1906.(in Chinese)
[20] 徐文丹.卵母细胞中丙酮酸脱氢酶激酶4的作用及其机制研究[D].苏州:苏州大学, 2019. XU W D.The function and mechanism of pyruvate dehydrogenase kinase 4 in oocytes[D].Suzhou:Suzhou University, 2019.(in Chinese)
[21] SIDRAT T, KHAN A A, JOO M D, et al.Bovine oviduct epithelial cell-derived culture media and exosomes improve mitochondrial health by restoring metabolic flux during pre-implantation development[J].International Journal of Molecular Sciences, 2020, 21(20):7589.
[22] 李忠秋, 刘春龙, 吴赛辉, 等.民猪丙酮酸脱氢酶激酶4基因在寒冷环境下的表达变化[J].黑龙江农业科学, 2020, 12:61-63. LI Z Q, LIU C L, WU S H, et al.Expression of PDK4 in Min pigs under cold environment[J].Heilongjiang Agricultural Science, 2020, 12:61-63.(in Chinese)
[23] 赵妤娟, 许生友, 姜润深, 等.热应激下鸡肝脏SFRP1和PDK4基因表达水平研究[J].中国家禽, 2012, 34(15):12-15. ZHAO Y J, XU S Y, JIANG R S, et al.Expression analysis of SFRP1 and PDK4 genes in livers of chicken under heat stress[J].China Poultry, 2012, 34(15):12-15.(in Chinese)
[24] 杨洋, 许厚强, 陈伟, 等.贵州从江香猪PDK4、FGF10基因的克隆及组织表达分析[J].中国畜牧兽医, 2017, 44(12):3401-3409. YANG Y, XU H Q, CHEN W, et al.Cloning and tissue expression analysis of PDK4 and FGF10 genes in Guizhou Congjiang Xiang pig[J].China Animal Husbandry & Veterinary Medicine, 2017, 44(12):3401-3409.(in Chinese)
[25] 潘鹏丞, 温斌华, 谢婉, 等.陆川猪PDK4基因序列分析、真核表达载体构建及组织表达分析[J].中国畜牧兽医, 2020, 47(8):2337-2347. PAN P C, WEN B H, XIE W, et al.Sequence analysis, eukaryotic expression vector construction and tissue expression analysis of PDK4 gene in Luchuan pig[J].China Animal Husbandry & Veterinary Medicine, 2020, 47(8):2337-2347.(in Chinese)
[26] 孙瑞萍, 王峰, 晁哲, 等.屯昌猪PDK4基因克隆及其组织表达分析[J].浙江农业学报, 2020, 32(6):978-985. SUN R P, WANG F, CHAO Z, et al.Cloning and tissue expression analysis of PDK4 gene in Tunchang pig[J].Acta Agriculturae Zhejiangensis, 2020, 32(6):978-985.(in Chinese)
[27] LAN J, LEI M G, ZHANG Y B, et al.Characterization of the porcine differentially expressed PDK4 gene and association with meat quality[J].Molecular Biology Reports, 2009, 36(7):2003-2010.
[28] WATHES D C, CHENG Z, SALAVATI M, et al.Relationships between metabolic profiles and gene expression in liver and leukocytes of dairy cows in early lactation[J].Journal of Dairy Science, 2021, 104(3):3596-3616.
[29] 唐立群, 肖层林, 王伟平.SNP分子标记的研究及其应用进展[J].中国农学通报, 2012, 28(12):154-158. TANG L Q, XIAO C L, WANG W P.Research and application progress of SNP markers[J].Chinese Agricultural Science Bulletin, 2012, 28(12):154-158.(in Chinese)
[30] 石福岳, 王燕燕, 容维中, 等.早胜牛CAPN1基因多态性及与肉质性状的关联性分析[J].中国畜牧杂志, 2021, 57(8):151-155. SHI F Y, WANG Y Y, RONG W Z, et al.Polymorphism of CAPN1 gene and its association with meat quality traits in Zaosheng cattle[J].Chinese Journal Animal Science, 2021, 57(8):151-155.(in Chinese)
[31] 张浩, 张亚楠, 李鑫, 等.PDK4对山羊肌内脂肪细胞脂代谢的影响[J].生物技术通报, 2021, 12:1-9. ZHANG H, ZHANG Y N, LI X, et al.Effects of PDK4 on lipid metabolism of goats intramuscular adipocytes[J].Biotechnology Bulletin, 2021, 12:1-9.(in Chinese)

Polymorphism of PDK4 Gene and Its Association with Meat Quality Traits in Chinese Steppe Red Cattle

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