松辽黑猪HBEGF基因多态性及其与繁殖性状的关联分析

doi:10.16431/j.cnki.1671-7236.2023.06.021

Abstract

Abstract: 【Objective】 The genetic diversity of single nucleotide polymorphism (SNP) of heparin-binding EGF-like growth factor (HBEGF) gene in Songliao Black pigs and its correlation with reproductive traits were analyzed.【Method】 The SNP of HBEGF gene were screened by Sanger direct sequencing in 90 healthy Songliao Black sows, and the correlation between SNP of HBEGF gene and reproductive traits (total number born, number born alive, birth weight, 3-week weight, weaning weight, weaning piglets and nipple number) were analyzed using SPSS 26.0 software.【Result】 A total of 22 SNPs were found in exon 5 and introns 2, 3, 4 and 5 of HBEGF gene in Songliao Black pigs, and a total of 8 SNPs were found to be significantly correlated with reproductive traits.Three genotypes of AA, AG and GG were contained in g.142114384 A>G and g.142104389_142104388ins A>G, three genotypes of CC, CA and AA were contained in g.142114375 C>A, two genotypes of CC and CT were contained in g.142111433 C>T, three genotypes of GG, GA and AA were contained in g.142106003 G>A and g.142105707_142105706ins G>A, three genotypes of CC, CG and GG were contained in g.142105965 C>G, and three genotypes of CC, CT and TT were contained in g.142105878_142105877ins C>T.The chi-square fitness test showed that g.142114375 C>A, g.142111433 C>T and g.142104389_142104388ins A>G were in Hardy-Weinberg equilibrium (P>0.05).g.142114384 A>G, g.142114375 C>A, g.142106003 G>A, g.142105878_142105877ins C>T and g.142104389_142104388ins A>G were moderate polymorphism (0.25<PIC<0.5), g.142111433 C>T, g.142105965 C>G and g.142105707_142105706ins G>A were low polymorphism (PIC<0.25).The results of association analysis showed that the total number born, number born alive and weaning piglets of AA genotype in g.142114384 A>G were significantly higher than those of GG genotype, the birth weight of GG genotype in g.142114384 A>G was significantly higher than that of AG genotype, and the nipple number of GG genotype in g.142114384 A>G was significantly higher than that of AA and AG genotypes (P<0.05).The total number born and number born alive of AA genotype in g.142114375 C>A were significantly higher than those of CC genotype, and the weaning piglets of AA genotype in g.142114375 C>A was significantly higher than that of CC and CA genotypes (P<0.05).The nipple number of CC genotype in g.142111433 C>T was significantly higher than that of CT genotype (P<0.05).The nipple number of GG genotype in g.142106003 G>A was significantly higher than that of AA genotype (P<0.05).The total number born, number born alive and weaning piglets of CC and GG genotypes in g.142105965 C>G were significantly higher than those of CG genotype (P<0.05).The weaning piglets of CC and TT genotypes in g.142105878_1421 05877ins C>T was significantly higher than that of CT genotype, and the nipple number of TT genotype in g.142105878_142105877 ins C>T was significantly higher than that of CC genotype (P<0.05).The 3-week weight and weaning weight of AA genotype in g.142105707_142105706ins G>A were significantly higher than that of GG genotype (P<0.05).The total number born and number born alive of AG genotype in g.142104389_142104388ins A>G was significantly higher than those of AA genotype (P<0.05).【Conclusion】 There were 8 SNPs of HBEGF gene that had significant correlation with total number born, number born alive, birth weight, 3-week weight, weaning weight, weaning piglets and nipple number of Songliao Black pigs.

Key words: Songliao Black pigs; HBEGF gene; reproductive performances; association analysis; genetic markers

CLC Number:

S813.3

ZHANG Fangwei, ZHANG Qi, LEI Liangliang, SUN Wusheng, ZHANG Di, ZHANG Yunpeng, ZHANG Jingbo, WANG Xiuquan, ZHANG Jing, ZHANG Shumin. Polymorphism of HBEGF Gene and Its Association with Reproductive Traits in Songliao Black Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2370-2379.

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Polymorphism of HBEGF Gene and Its Association with Reproductive Traits in Songliao Black Pigs

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