基于高通量测序技术挖掘分子遗传标记及其在蛋鸡生产中的精准应用

doi:10.16431/j.cnki.1671-7236.2022.10.019

Abstract

Abstract: High-throughput sequencing (HTS) is the basis for studying the genetic mechanisms of complex biological traits.With the continuous optimization and improvement of high-throughput sequencing technology,some genomic variations related to biological phenotype traits have been accurately excavated,including single nucleotide polymorphism (SNP),insertion/deletion (Indel),copy number variation (CNV),and structure variation (SV) as the representative molecular markers.Compared with the traditional breeding method,molecular genetic markers exhibit high polymorphism throughout the genome.The detection method is simple,quick,and economic.By detecting genome-wide molecular markers and using genome-wide genetic information to assess individual or population genetic resources,the generation interval can be shorten and the accuracy of seed selection can be improved,thus achieving great genetic progress in a short period of time.From the perspective of high-throughput sequencing technology mining molecular genetic markers,this paper summarizes the development history and application fields of third-generation sequencing technology and the application of third-generation molecular genetic marker detection technology in the innovation of laying hens breeding industry.Moreover,The precise application of high-throughput sequencing technology combined with molecular genetic markers in genetic diversity and evolutionary classification of laying hens,construction of population genetic map and mapping of functional genes,genetic mechanism analysis of quantitative traits and genetic mechanism analysis of quality traits were discussed in detail,in order to provide scientific basis and guidance for laying hens genome selection into practical application stage.

Key words: high-throughput sequencing technology; molecular genetic markers; laying hens production; accurate application

CLC Number:

ZHAO Jibo, TAN Lei, PAN Hongbin, HUANG Chang, LIU Yong, WANG Guangzheng, DOU Tengfei, WANG Kun, LI Zijian, GE Changrong. Mining Molecular Genetic Markers Based on High-throughput Sequencing Technology and Its Accurate Application in Laying Hens Production[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3866-3878.

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Mining Molecular Genetic Markers Based on High-throughput Sequencing Technology and Its Accurate Application in Laying Hens Production

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