超氧化物歧化酶模拟物对氧化应激IPEC-J2细胞抗氧化性能的影响

doi:10.16431/j.cnki.1671-7236.2021.05.007

Abstract

Abstract: The objective of this study was to investigate the protective effect of superoxide dismutase mimetic (SODm) on intestinal epithelial cell (IPEC-J2) under H₂O₂-induced oxidative stress.Using MTT method to screen out the proper concentration of H₂O₂ applied in structuring oxidative stress model.IPEC-J2 were cultured with 0,0.05,0.5,5,50,500 and 2 000 U/mL SODm,respectively.The survival of each group was detected by MTT in 2,4,8 and 12 h to determine the proper range and duration of a SODm dose.Based on the model of oxidative stress and the proper range and duration of SODm,IPEC-J2 were randomly grouped as the control group,the model group with H₂O₂,the disposed group with SODm (SODm_0.5,SODm₅,SODm₅₀),the disposed group with SOD (SOD_0.5,SOD₅,SOD₅₀),then cell viability,ROS levels and SOD,GSH-Px,MDA,T-AOC levels in cells were measured in each group.The results were showed as follows:①1.0 mmol/mL H₂O₂ was the proper concentration in structuring oxidative stress model.②The survival ratios of the disposed group with SODm of 0.5~50 U/mL in 4,8 and 12 h was significantly higher than that of the control groups (P<0.05).The survival ratio was highest at 8 h and decreased at 12 h.③The IPEC-J2 survival rate of the SODm₅ and SOD₅ groups were significantly higher than that in other groups (P<0.05) except for the blank group,and the ROS contents in the SODm and SOD groups were significantly lower than that in the model group (P<0.05).The activities of SOD,GSH-Px and T-AOC were significantly decreased,and MDA content were significantly increased in model group compared with the control group (P<0.05).The treatments of SODm and SOD increased the activities of SOD,GSH-Px and T-AOC,and significantly decreased MDA content compared with the model group(P<0.05),The T-AOC level of SOD₅₀ group was higher than that of SODm₅₀ group (P<0.05).In conclusion,SODm had protective effect on IPEC-J2 cells under H₂O₂-induced oxidative stress.

Key words: superoxide dismutase mimetic(SODm); oxidative stress; IPEC-J2 cells

CLC Number:

WANG Sibo, CUI Hongxia, YANG Ji, XU Li. Effect of Superoxide Dismutase Mimetic on Antioxidant Capacity of Oxidatively Damaged IPEC-J2 Cells[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1566-1573.

References

[1] SANCHEZ ALVAREZ M,STRIPPOLI R,DONA-DELLI M,et al.Sestrins as a therapeutic bridge between ROS and autophagy in cancer[J].Cancers,2019,11(10):1415.
[2] 齐鸣,谭碧娥.自噬调节动物氧化应激反应的分子机制[J].动物营养学报,2020,32(9):3993-4002. QI M,TAN B E.Molecular mechanism of autophagy regulating oxidative stress in animals[J].Chinese Journal of Animal Nutrition,2020,32(9):3993-4002.(in Chinese)
[3] 陈凤鸣,陈佳亿,彭伟,等.氧化应激对猪肠道损伤机制的研究进展[J].动物营养报,2018,30(8):2887-2893. CHEN F M,CHEN J Y,PENG W,et al.Research progress of oxidative stress on mechanism of intestinal damage in pigs[J].Chinese Journal of Animal Nutrition,2018,30(8):2887-2893.(in Chinese)
[4] 朱丽慧.早期断奶仔猪肠细胞凋亡分子机理及营养调控研究[D].上海:上海交通大学,2014. ZHU L H.Molecular mechanism of apoptosis in weaned piglets and nutrition regulation[D].Shanghai:Shanghai Jiao Tong University,2014.(in Chinese)
[5] SMITH F,CLARK J E,OVERMAN B L,et al.Early weaning stress impairs development of mucosal barrier function in the porcine intestine[J].American Journal of Physiology-Gastrointestinal and Liver Physiology,2010,298(3):G352-G363.
[6] HU C H,XIAO K,LUAN Z S,et al.Early weaning increases intestinal permeability,alters expression of cytokine and tight junction proteins,and activates mitogen-activated protein kinases in pigs[J].Journal of Animal Science,2013,91(3):1094-1101.
[7] FRESE S A,PARKER K,CALVERT C C,et al.Diet shapes the gut microbiome of pigs during nursing and weaning[J].Microbiome,2015,28(3):1-10.
[8] GUEVARRA R B,LEE J H,LEE S H,et al.Piglet gut microbial shifts early in life:Causes and effects[J].Journal of Animal Science and Biotechnology,2019,10(1):1-10.
[9] WANG Q,NIE P,HOU Y,et al.Purification,biochemical characterization and DNA protection against oxidative damage of a novel recombinant superoxide dismutase from psychrophilic bacterium Halomonas sp.ANT108[J].Protein Expression Puri-fication,2020,173:105661.
[10] 蒋华梅,王向前.插田泡中超氧化物歧化酶的提取纯化及鉴定[J].食品工业,2018,39(9):81-85. JIANG H M,WANG X Q.Purification and identification of superoxide dismutase from Rubus coreanus Maq.Leaves[J].The Food Industry,2018,39(9):81-85.(in Chinese)
[11] 张娜,赵倩芸,刘锰钰,等.植物中超氧化物歧化酶的提取及应用[J].中国洗涤用品工业,2017,8:87-90. ZHANG N,ZHAO Q Y,LIU M Y,et al.The extraction and application of SOD in plants[J].China Cleaning Industry,2017,8:87-90.(in Chinese)
[12] 王艳红,秦峰,董皎,等.锰超氧化物歧化酶模拟化合物对人胃癌MGC-803细胞增殖和凋亡的影响[J].中国新药与临床杂志,2017,36(3):141-145. WANG Y H,QIN F,DONG J,et al.Effects of manganese superoxide dismutase mimic on prolifera-tion and apoptosis of human gastric carcinoma MGC-803 cells[J].Chinese Journal of New Drugs and Clinical Remedies,2017,36(3):141-145.(in Chinese)
[13] 王艳红,冯铁新,蒲君峰,等.锰超氧化物歧化酶模拟物对四氯化碳致小鼠急性肝损伤的保护作用[J].中国临床药理学与治疗学,2018,23(11):1209-1214. WANG Y H,FENG T X,PU J F,et al.Protective effects of manganese superoxide dismutase mimic on liver injury in mice induced by carbon tetra-chloride[J].Chinese Journal of Clinical Pharmacology and Therapeutics,2018,23(11):1209-1214.(in Chinese)
[14] 王鑫瑶,赵鑫淼.茶多酚的提取技术及优缺点比较研究进展[J].广州化工,2018,46(8):26-27. WANG X Y,ZHAO X M.Research progress on extraction technology and its advantages and disadvan-tages of tea polyphenol[J].Guangzhou Chemical Industry,2018,46(8):26-27.(in Chinese)
[15] SUN X F,FU X,DU M,et al.Ex vivo gut culture for studying differentiation and migration of small intestinal epithelial cells[J].Royal Society Open Biology,2018,8(4):170256.
[16] MOHAMED A E,YASMIN A A,CRAIG H,et al.The novel SOD mimetic GC4419 increases cancer cell killing with sensitization to ionizing radiation while protecting normal cells[J].Free Radical Biology and Medicine,2020,160:630-642.
[17] SANTIANI A,EVANGELISTA S,SEPULVEDA N,et al.Addition of superoxide dismutase mimics during cooling process prevents oxidative stress and improves semen quality parameters in frozen/thawed ram spermatozoa[J].Theriogenology,2014,82(6):884-889.
[18] SAKR O G,GAD A,RODRIGUEZ M E,et al.Superoxide dismutase mimics improves semen quality during chilled preservation of rabbit spermatozoa[J].Livestock Science,2019,211,70-76.
[19] GEENS M M,NIEWOLD T A.Optimizing culture conditions of a porcine epithelial cell line IPEC-J2 through a histological and physiological characteri-zation[J].Cytotechnology,2011,63(4):415-423.
[20] LEE J C,SON Y O,Choi K C,et al.Hydrogen peroxide induces apoptosis of BJAB cells due to formation of hydroxyl radicals via intracellular iron-mediated Fenton chemistry in glucose oxidase-mediated oxidative stress[J].Molecules and Cells,2006,22(1):21-29.
[21] ESSICK E E,WILSON R M,PIMENTEL D R,et al.Adiponectin modulates oxidative stress-induced autophagy in cardiomyocytes[J].PLoS One,2013,8(7):e68697.
[22] 金鹿,闫素梅,史彬林,等.过氧化氢诱导的奶牛乳腺上皮细胞氧化损伤模型的建立[J].动物营养学报,2014,26(12):3651-3658. JIN L,YAN S M,SHI B L,et al.Establishment of oxidative damage model of bovine mammary epithelial cells induced by hydrogen peroxide[J].Chinese Journal of Animal Nutrition,2014,26(12):3651-3658.(in Chinese)
[23] 谭秀文,游伟,刘晓牧,等.肉牛肌肉细胞的分离培养和鉴定及其氧化应激模型的建立[J].动物营养学报,2020,32(5):2369-2378. TAN X W,YOU W,LIU X M,et al.Isolation,culturing and identification of beef cattle muscle cells and establishment of its oxidative stress model[J].Chinese Journal of Animal Nutrition,2020,32(5):2369-2378.(in Chinese)
[24] 张润蔚,王玲,张春晓,等.过氧化氢诱导斜带石斑鱼原代肝细胞氧化损伤模型的构建[J].动物营养学报,2017,29(4):1227-1232. ZHANG R W,WANG L,ZHANG C X,et al.Establishment of oxidative damage model of primary hepatocytes of grouper (Epinephelus coioides) induced by gydrogen peroxide[J].Chinese Journal of Animal Nutrition,2017,29(4):1227-1232.(in Chinese)
[25] CAI X,CHEN X,WANG X,et al.Pre-protective effect of lipoic acid on injury induced by H₂O₂ in IPEC-J2 cells[J].Molecular and Cellular Biochemistry,2013,378(1-2):73-81.
[26] 肖定福,钟佳,刘进辉,等.壳寡糖对脂多糖诱导猪空肠上皮细胞氧化损伤的作用[J].动物营养学报,2016,28(7):2090-2095. XIAO D F,ZHONG J,LIU J H,et al.Effects of chitooligosaccharide on lipopolysaccharide induced oxidative damage in epithelial cells of pig jejunum[J].Chinese Journal of Animal Nutrition,2016,28(7):2090-2095.(in Chinese)
[27] 邹轶.止痢草油在缓解运输应激诱导的肠道氧化损伤中的作用及机制研究[D].武汉:华中农业大学,2017. ZOU Y.The mitigation role of dietary oregano essential oil in intestinal oxidative stress induced by transportation[D].Wuhan:Huazhong Agricultural University,2017.(in Chinese)
[28] ZHU L H,ZHAO K L,CHEN X L,et al.Impact of weaning and an antioxidant blend on intestinal barrier function and antioxidant status in pigs[J].Journal of Animal Science,2012,90(8):2581-2589.
[29] MILLER M J,MCNEILL H,MULLANE K M,et al.SOD prevents damage and attenuates eicosanoid release in a rabbit model of necrotizing enteroco-litis[J].The American Journal of Physiology,1988,255(5Pt1):G556-G565.
[30] DROY LEFAIX M T,DROUET Y,GERAUD G,et al.Superoxide dismutase(SOD) and the PAF-antagonist(BN 52021) reduce small intestinal damage induced by ischemia-reperfusion[J].Free Radical Research,2009,13(1):725-735.
[31] ZAIB UR R,MENG C,SUN Y,et al.Oxidative stress in poultry:Lessons from the viral infections[J].Oxidative Medicine and Cellular Longevity,2018,2018,1-14.
[32] FLOHE L,GUNZLER W A,SCHOCK H H.Glutathione peroxidase:A selenoenzyme[J].FEBS Letters,1973,32(1):132-134.
[33] 张鹏,刘琰,李萌,等.高糖环境下锰超氧化物歧化酶转染间充质干细胞促进抗氧化酶类表达[J].中国现代医学杂志,2014,24(20):27-30. ZHANG P,LIU Y,LI M,et al.Manganese superoxide dismutase transfers the mesenchymal stem cells to promote the expression of antioxidant enzyme in the high glucose conditions[J].China Journal of Modern Medicine,2014,24(20):27-30.(in Chinese)
[34] 王伟,彭昊.穿透肽介导的铜-锌超氧化物歧化酶1融合蛋白的抗氧化作用及对脂肪干细胞移植修复脊髓损伤的促进作用[J].中华实验外科杂志,2017,34(10):1721-1724. WANG W,PENG H.Antioxidative effect of copper-zinc superoxide dismutase 1 fusion protein mediated by penetrating peptide and promotion effect of adipose stem cell transplantation for spinal cord injury repair[J].Chinese Journal of Experimental Surgery,2017,34(10):1721-1724.(in Chinese)

Effect of Superoxide Dismutase Mimetic on Antioxidant Capacity of Oxidatively Damaged IPEC-J2 Cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SUN Chen, GE Sheng, NING Xiaqing, WANG Jiaqi, ZHANG Nuannuan, WANG Xiaoran, ZHANG Shixia. Oxidative Stress Model of BRL-3A Cells Induced by Sodium Hydrosulfite [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2217-2223.
[2]	WANG Jing, SONG Hongbing, WU Yanling, LIN Weimin, XIAO Tianfang. Research Progress of Crocin in Animal Antioxidants [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2233-2244.
[3]	LI Rui, ZHANG Yiyang, LUO Xuehui, ZHOU Manlin, WU Shaomi, YANG Kun, QIAO Zilin. Effect of Hypoxia on Proliferation and Oxidative Stress Injury of Pulmonary Artery Smooth Muscle Cells in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2245-2254.
[4]	LI Yongqiang, BAO Ruiying, WANG Qin, QIU Pingfei, LI Xiaochun, SHI Huiyu, ZHANG Haiwen, WANG Xuemei. Protective Effects of Antioxidant Peptides from Maggot on Oxidative Stress Injury of IPEC-J2 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1836-1844.
[5]	ZHANG Jinwu, WEI Zi, JI Yutong, WU Changxu, LI Zhenmin, WEI Yingyi, HU Tingjun, YU Meiling. Study on Oxidative Stress and Inflammatory Injury in Intestinal Tract of Yellow-feathered Chickens Induced by Clostridium perfringens Type A [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2069-2081.
[6]	DOU Jiahong, WANG Ziying, YANG Juan, ZHOU Weiwei, ZHANG Tongcun, HE Hongpeng, DAI Xiaofeng, LI Xiumei. Analysis of the Active Ingredients and Mechanism of Radix Paeoniae Rubra Antioxidative Stress Based on Network Pharmacology and Molecular Docking [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1229-1240.
[7]	CHEN Jin, XIE Jinghao, HAN Yingqian, YANG Yanbin, WANG Yueying, LI Heping. Anti-inflammatory and Antioxidant Effects of Euphorbiae humifusae Aqueous Extract in IPEC-J2 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 704-712.
[8]	LIU Bo, LI Meilin, LIU Xu, LIANG Shuangying, MU Baolong, ZHAO Wenting, GE Yansong. Effects of Adipose-derived Stem Cell Conditioned Medium Alleviates Oxidative Stress Injury Induced by Sodium Taurocholate and Trypsin [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 798-806.
[9]	FU Jingcheng, HE Junhui, TONG Jiang, WU Wei, GUO Shuang, YANG Yanbin, HAN Yingqian, WANG Yueying, LI Heping. Anti-inflammatory and Antioxidant Roles of HO-1 in Macrophages [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 807-816.
[10]	MIAO Weiyuan, ZHANG Siqi, HUANG Changqiao, ZHAO Yi, CHEN Qi, XIE Xiaodong, WEI Yingyi, YU Meiling, HU Tingjun. Effect of N-butanol Fraction of Polygonum hydropiper Flavonoids on Levels Oxidative Stress-related Factors in 3D4/2 Cells Infected with PRV [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 349-358.
[11]	ZHANG Zufeng, ZHANG Yuxin, SUN Yuelong, ZHENG Wei, LI Xiumei. Study on Mechanism of Hawthorn in Regulating Oxidative Stress Based on Network Pharmacology and Molecular Docking [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 408-420.
[12]	LU Wengeng, SI Linqing, TIAN Chunyu, YUAN Siqi, XIE Hexin, ZHOU Jiwei, JIN Jidong, HAN Yinghao. Establishment of Oxidative Stress Model of Bovine Endometrial Epithelial Cells and the Effects of Bovine Adipose-derived Mesenchymal Stem Cells on Cell Migration [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2506-2514.
[13]	WANG Shuang, LIU Xingyao, JIA Haotian, LIU Yun. Alleviative Effect of Hydrogen Sulfide on Cisplatin-induced Kidney Oxidative Damage and Inflammation in Dogs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2376-2384.
[14]	YANG Yu, GUAN Wenchao, CUI Xiaozhen, JIA Yongxin, YANG Shiyu. Effects of Licochalcone A on Oxidative Stress Induced by Haemophilus parasuis Infection in Porcine Alveolar Macrophages [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 480-487.
[15]	JIAO Anhui, ZHANG Xiaomeng, ZHAO Yuhan, WANG Yu, GAO Qingshan. Effects of Limonin on in vitro Maturation of Mouse Oocytes and Embryo Development of in vitro Fertilization [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3891-3900.