甘草查尔酮A对副猪嗜血杆菌感染猪肺泡巨噬细胞氧化应激的影响

doi:10.16431/j.cnki.1671-7236.2022.02.009

Abstract

Abstract: 【Objective】 This study was to explore the effects of licochalcone A (Lico A) on the oxidative stress of porcine alveolar macrophages (PAMs) infected with Haemophilus parasuis, so as to provide reference for the development of new drugs for the treatment of Haemophilus parasuis infection.【Method】 PAMs were obtained from the lungs by bronchoalveolar lavage and divided into 6 groups. Negative control group, PAMs were not infected with Haemophilus parasuis; Positive control group, PAMs were infected with Haemophilus parasuis only; DMSO group, PAMs were treated with 0.1% DMSO after infection with Haemophilus parasuis; 5, 10 and 20 μg/mL Lico A group, PAMs were treated with 5, 10 and 20 μg/mL Lico A after infection with Haemophilus parasuis.PAMs were treated according to the above groups and cultured for 24 h.Cell viability were measured by CCK-8 method, the activities of lactate dehydrogenase (LDH), glutathione peroxidase(GSH-Px)and superoxide dismutase (SOD), and the levels of malondialdehyde (MDA), nitric oxide (NO) and total antioxidant capacity (T-AOC) in the supernatant were measured by enzyme labeling or visible light photometer, respectively.The intracellular reactive oxygen species (ROS) level was observed using a fluorescence probe, 2', 7'-dichlorofluorescin diacetate (DCFH-DA).【Result】 Compared with negative control group, after infection with Haemophilus parasuis, the activity of PAMs was decreased significantly (P < 0.05), the activity of LDH in supernatant was significantly increased (P < 0.05), the levels of MDA, NO and ROS and the activity of SOD were significantly increased (P < 0.05).Compared with positive group, the cell viability in 5, 10 and 20 μg/mL Lico A groups was significantly increased (P < 0.05), the activity of LDH in supernatant was significantly decreased (P < 0.05), the levels of MDA, NO and ROS in 10 and 20 μg/mL Lico A groups were significantly decreased (P < 0.05).The activities of GSH-Px, SOD and T-AOC in 5, 10 and 20 μg/mL Lico A groups were significantly increased in a dose dependent manner (P < 0.05).【Conclusion】 Haemophilus parasuis infection results in an imbalance between oxidant and antioxidant in PAMs, and 5-20 μg/mL Lico A could decrease the cellular response to oxidative stress.

Key words: licochalcone A; Haemophilus parasuis; porcine alveolar macrophages; oxidative stress

CLC Number:

S853.74

YANG Yu, GUAN Wenchao, CUI Xiaozhen, JIA Yongxin, YANG Shiyu. Effects of Licochalcone A on Oxidative Stress Induced by Haemophilus parasuis Infection in Porcine Alveolar Macrophages[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 480-487.

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Effects of Licochalcone A on Oxidative Stress Induced by Haemophilus parasuis Infection in Porcine Alveolar Macrophages

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