副猪嗜血杆菌血清7型菌株毒力及耐药性分析

doi:10.16431/j.cnki.1671-7236.2022.08.040

Abstract

Abstract: 【Objective】 This study was aimed to understand the virulence and drug resistance of Haemophilus parasuis (Hps) serotype 7 epidemic strains in Henan province.【Method】 Taking the reference strain of Hps serotype 7 as the control,the 16S rRNA gene amplification,sequence analysis,virulence gene detection,pathogenicity test and drug sensitivity test were carried out on 6 strains of Hps serotype 7 isolated and identified from clinical cases.【Result】 16S rRNA gene sequencing and similarity comparison showed that the similarity between strain 1436 and standard strain 0007 was 100%,and that between strain 1565 and 1624 and standard strain were 98.6%. Phylogenetic tree analysis showed that 1436 had the closest genetic relationship with standard strain 0007,1624 was the farthest related to standard strain 0007.Six clinical strains and standard strain showed two virulence genotypes.All strains carried vta1,vta2, vta3,wza,nanH,cdtA,cdtB,cdtC and espP2 virulence genes,and five clinical strains also carried ompP2 virulence gene.In the guinea pig pathogenicity test,the clinical strain showed different degrees of virulence enhancement compared with the reference strain.The reference strain could not infect guinea pigs and did not show any clinical symptoms.The clinical isolates could infect guinea pigs,showing some clinical symptoms,the incidence rates were 20% to 40%,and the mortality rate was 0.The standard strain and clinical strains were sensitive to ceftazidime and ceftiofur,they were mediated to doxycycline and florfenicolo,the resistance to antibiotics such as neomycin,kanamycin,amikacin,erythromycin and tilmicosin was strong,and all strains showed multiple drug resistance.【Conclusion】 Hps serotype 7 clinical strains had certain pathogenicity,but their virulence was weak,they were sensitive to cephalosporins and had obvious multi drug resistance.Clinically,the prevention and control of this serotype should be paid attention.This study laid a foundation for the epidemiology and pathogenesis of Hps serotype 7,and provided a reference basis for the clinical prevention and control of Hps serotype 7.

Key words: Haemophilus parasuis (Hps); serotype 7; typing and identification; virulence genes; pathogenicity test; drug sensitivity test

CLC Number:

S852.61⁺2

WANG Zhifang, XU Yindi, ZHU Wenhao, ZHANG Qingxian, JIAO Wenqiang, LI Haili, WANG Keling. Analysis of Virulence and Drug Resistance of Haemophilus parasuis Serotype 7 Strains[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3245-3255.

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Analysis of Virulence and Drug Resistance of Haemophilus parasuis Serotype 7 Strains

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