浣熊源粪肠球菌的分离鉴定与耐药性分析

doi:10.16431/j.cnki.1671-7236.2021.03.036

Abstract

Abstract: To investigate the characteristics and antimicrobial resistance of a dominant bacterium isolated from the liver of a dead raccoon in a zoo in Sichuan province.In this study,Gram staining,biochemical identification,16S rRNA sequence determination,pathogenicity test,antimicrobial susceptibility test and resistance gene amplification test were used to conduct morphological,biochemical identification and study on pathogenicity and drug resistance of the isolated strain.The result showed that the isolated strain showed a translucent colony with smooth surface,round protrusion,neat edge and needle tip size on LB medium.Blue-purple cocci could be observed with Gram staining under oil microscope.The isolate was 99.5% similar to Enterococcus faecalis whose GenBank accession No. was KY003107.1,and the morphological and biochemical characteristics were consistent with those of Enterococcus faecalis.The pathogenicity test showed that the bacterium could cause hepatocytes swelling and degeneration,and had certain pathogenicity.Antimicrobial susceptibility test and resistance gene amplification test showed that the strain was resistant to 14 common antibiotics,cefoxitin,cefoperazone,ceftriaxone,cefepime,cefotaxime,ampicillin,kanamycin,streptomycin,netimicin,butanicin,azithromycin,erythromycin,doxycycline and tetracycline,and was only moderately sensitive to vancomycin and gentamicin.Tetracycline resistance gene tetC was positive,tetM and tetA gene were negative.Aminoglycoside resistance gene aac(6')/aph(2″) was positive,aph(3')-Ⅲ and ant(6)-Ⅰ were negative.The ermB of macrolide resistance genes was positive and ermA and ermC were negative.Vancomycin resistance gene vanA,vanB and β-lactam resistance gene TEM were all negative.In this study,the Enterococcus faecalis isolated from raccoons showed high drug resistance and pathogenicity,which could be used as a reference for the selection of drugs for clinical Enterococcus faecalis infection,and a reasonable infection prevention and treatment scheme could be formulated.

Key words: Enterococcus faecalis; biochemical identification; 16S rRNA; pathogenicity test; antimicrobial susceptibility test; resistance genes

CLC Number:

Q93-337

ZHONG Rui, HE Tingmei, BI Bo, YANG Xingtao, SHI Xiaogang, JIN Senlong, FENG Gang, PENG Guangneng. Isolation,Identification and Drug Resistance Analysis of Enterococcus faecalis from Raccoon[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1112-1120.

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Isolation,Identification and Drug Resistance Analysis of Enterococcus faecalis from Raccoon

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