育肥猪粪便菌群结构及其与生长速度的相关性分析

doi:10.16431/j.cnki.1671-7236.2022.03.014

Abstract

Abstract: 【Objective】 To study the relationship between the growth rate of growing finishing pigs and fecal microbiota, and find the microbial flora related to the growth characteristics of pigs.【Method】 50 piglets with similar birth weights were selected and carried out feeding and management in the same feeding environment, and transfered sick pigs to the experimental group in time.At the age of 125 days, 4 pigs with the highest and lowest body weight were divided into high body weight (51.30 kg±2.57 kg, HW) and low body weight (36.90 kg±2.50 kg, LW) groups.The fecal microflora diversity, colony composition and correlation with growth rate of 125-day-old growing finishing pigs were analyzed by 16S rRNA sequencing.【Result】 Alpha diversity analysis showed that there was no significant difference between HW and LW groups (P>0.05).PCA results showed that it could clearly distinguish the flora structure in HW and LW groups.The analysis of the structure and composition of fecal microbiota showed that the dominant phyla in HW and LW groups were Firmicutes, Bacteroidetes and Spirochaetes, and the dominant genus were Treponema_2, Lactobacillus, Streptococcus and uncultured_bacterium_f_Muribaculaceae.The abundance of Bacteroides and Fibrobacteres in LW group was extremely significantly or significantly higher than that in HW group (P<0.01 or P<0.05), and Patescibacteria was significantly lower than that in HW group (P<0.05).The difference analysis of the fecal microbial structure and composition showed that 16 unique bacteria phyla were detected in HW group, 1 unique phyla and 24 unique genera were detected in LW group.The resuts of the correlation between intestinal flora and growth rate showed that 10 bacterial phyla and 18 genera were positively correlated with body weight (BW) and average daily gain (ADG), 7 bacteria phyla and 12 genera were negatively correlated with BW and ADG.【Conclusion】 There were significant differences in the flora of pig fecal microbiota with different growth rates at the same age, and the different flora might have a certain regulatory effect on the growth rate of pigs.This results could provide a reference for the development of probiotics and the improvement of the growth rate of growing pigs.

Key words: pigs; fecal microbiota; growth rate; 16S rRNA sequencing

CLC Number:

S816.3

JIANG Changjin, CHEN Yun, PAN Pengcheng, CHEN Baojian, GUAN Zhihui, LU Huilin, XIE Bingkun, QIN Zhaoxian. Fecal Microflora Structure and Its Correlation with Growth Rate in Finishing Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 924-931.

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Fecal Microflora Structure and Its Correlation with Growth Rate in Finishing Pigs

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