猪胎盘营养转运相关基因在不同妊娠期的表达

doi:10.16431/j.cnki.1671-7236.2022.08.022

Abstract

Abstract: 【Objective】 The aim of this study was to explore the expression patterns of amino acids,glucose and fatty acid transporters in porcine placenta at different gestation periods.【Method】 15 Duroc sows with similar genetic background and litter size were randomly divided into 3 groups.After estrus,all sows were artificially inseminated with semen collected from the same Duroc boar.The uteruses of sows in each group were taken out by anesthesia on day 40 (D40),65 (D65) and 95 (D95) of gestation.The uterus was quickly opened to separate the placental tissue of each fetus and the total RNA of placental tissue was extracted and synthesized cDNA.The synthesized primers were used for general PCR amplification and 2.0% agarose gel was used to detect the amplification products.Real-time quantitative PCR was used to detect and compare the relative mRNA expression levels of amino acid,glucose,fatty acid transporters associated genes in placenta at three pregnancy stages.【Result】 PCR results showed that the length of amino acid transporter-related genes(SLC7A1,SLC7A2,SLC7A3,SLC7A4,SLC7A10,SLC1A3,SLC1A5,SLC38A10,SLC36A1),glucose transporter related genes(SLC2A1,SLC2A2,SLC2A3,SLC2A10,SLC2A12,SLC2A13),and fatty acid transporter-related genes (FATP1,FATP2,FATP3,FATP4,FABP3,FABP7,CD36) were consistent with the expected.Real-time quantitative PCR results showed that in amino acid transporters,the expression of SLC7A4,SLC7A10 and SLC38A10 genes in D65 placenta were significantly higher than those in D40 placenta (P<0.05),while SLC7A2 gene was significantly lower than that in D40 placenta (P<0.05),and the expression SLC1A3 and SLC7A4 genes in D65 placenta were significantly lower than those in D95 placenta (P<0.05).In glucose transporters,the expression of SLC2A3 and SLC2A13 genes in D65 and D95 placenta were significantly higher than those in D40 placenta,while the expression of SLC2A1,SLC2A2 and SLC2A12 genes in D95 placenta were significantly lower than those in D65 placenta (P<0.05).In fatty acid transporters,the expression of FATP2,FATP4,FABP3,FABP5,FABP7 and CD36 genes in D65 placenta were significantly higher than those in D40 placenta(P<0.05),while the expression of FATP1, FATP4 and CD36 genes were significantly lower than those in D95 placenta (P<0.05).【Conclusion】 The placental SLC7A10,SLC38A10,SLC7A4,SLC2A3,FATP1,FATP4,FABP7 and CD36 might be nutrition transport-related genes affecting fetal growth and development in porcine pregnancy.

Key words: pigs; placenta; nutrition transfer; growth and development

CLC Number:

S814.4

WU Zhimin, HU Guangling, AO Zheng. Expression of Nutrition Transport-related Genes in Porcine Placenta at Different Gestation Periods[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3062-3071.

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Expression of Nutrition Transport-related Genes in Porcine Placenta at Different Gestation Periods

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