大型迪庆藏猪不同生长阶段肌内脂肪沉积差异表达基因及其调控通路分析

doi:10.16431/j.cnki.1671-7236.2022.08.002

Abstract

Abstract: 【Objective】 This study was aimed to screen the key genes of the difference of intramuscular fat (IMF) content of large Diqing Tibetan pigs (TPs) at different growth stages and analyze its regulation pathway.【Method】 There were thirty-six TPs with the same parity,date of birth and weight were randomly divided into three groups.The fattening test was carried out under the same conditions.They were slaughtered when the weight was about 40,80 and 120 kg,respectively.The longissimus dorsi muscle (LD) of three pigs in each group was collected,and the transcriptome was sequenced by RNA-Seq.The sequencing data was spliced,compared and annotated,the significantly differentially expressed genes (DEGs) related to IMF deposition were screened,and the gene interaction network was constructed by functional enrichment and STEM analysis.The results were verified by Real-time quantitative PCR.【Result】 There were 730,981 and 735 genes were significantly differentially expressed in 40 kg vs 80 kg,80 kg vs 120 kg and 40 kg vs 120 kg stages,respectively.There were four modules with significant differences in STEM analysis.The gene sets of modules 11 and 14 were significantly up-regulated and then slightly down-regulated.The gene sets of modules 9 and 10 were significantly up-regulated and then down-regulated.The differential gene interaction network showed that at 40 kg vs 80 kg stage,EGR1,EGR2,PRKAG2,NOR-1 and ATF3 genes were located in the core of the network,EGR1 and EGR2 genes were down-regulated,PRKAG2,NOR-1 and ATF3 genes were up-regulated.At 80 kg vs 120 kg stage,FOXO1,PDK4,PPARD,PPARGC-1,LIPE,ATF3 and STAT1 genes were located in the core of the network,FOXO1,PPARD and PPARGC-1 genes were down-regulated,and PPARG gene caused STAT1 gene down-regulation through cascade regulation,LIPE and ATF3 genes were up-regulated.At 40 kg vs 120 kg stage,ATF3,NOR-1,EGR1,EGR2 and STAT1 genes were located in the core of the network,EGR1,EGR2 and STAT1 genes were down-regulated,ATF3 and NOR-1 genes were up-regulated.Real-time quantitative PCR results of ten genes such as ATF3 and FOXO1 were consistent with RNA-Seq results.【Conclusion】 This study screened some genes like EGR1 and FOXO1 participate in the regulation of IMF in TPs as core genes,which involved in regulation at different stages were not exactly the same.The results could enrich the basic data of IMF regulation in local pigs in China,and provide a reference for the genetic improvement of IMF content in TPs.

Key words: large Diqing Tibetan pigs; RNA-Seq; intramuscular fat deposition; differentially expressed gene; regulation pathway

CLC Number:

S828

NIE Jingru, MA Li, YAN Dawei, DENG Jun, ZHANG Hao, ZHANG Bo, LIU Jinqiao, DONG Xinxing. Analysis of Differentially Expressed Genes and Regulation Pathways of Intramuscular Fat Deposition in Large Diqing Tibetan Pigs at Different Growth Stages[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2855-2868.

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Analysis of Differentially Expressed Genes and Regulation Pathways of Intramuscular Fat Deposition in Large Diqing Tibetan Pigs at Different Growth Stages

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