鸭瘟病毒感染鸭十二指肠黏膜炎症相关基因及调控通路的筛选

doi:10.16431/j.cnki.1671-7236.2020.10.032

Abstract

Abstract: This experiment was aimed to analyze the changes in the duodenal mucosal transcriptome level of duck plague virus (DPV) infected Cherry Valley ducks,and screen out genes and regulatory pathways involved in inflammation after DPV infection.DEV-GZ strain was used to inoculate 35 days old ducks with leg muscles,and the samples of duck duodenal mucosa tissues were collected at 24,48 and 72 h after inoculation,total RNA was extracted,and transcriptome sequencing was performed.Quality control and annotation of data were performed to screen differentially expressed genes and regulatory pathways related to inflammation,and some of the differentially expressed genes were verified by Real-time quantitative PCR.The results showed that a total of 21.39 Gb Clean Bases was obtained in control and experiment groups,the effective sequence of sequencing samples accounted for more than 99% of the original sequence,and the mapping rate was higher than 83%.There were 221 differentially expressed genes in duck duodenal mucosa after exposure 24 h with DEV-GZ,and 3 genes involved in inflammation-related biological processes,all of which were down-regulated genes.499 differentially expressed genes in duck duodenal mucosa after exposure 48 h with DEV,and 17 genes involved in inflammation-related biological processes,all of which were all down-regulated genes.798 differentially expressed genes in duck duodenal mucosa after exposure 72 h with DEV-GZ,and 20 were involved in inflammation-related biological processes,including 13 up-regulated genes and 7 down-regulated genes.GO database analysis showed that the differentially expressed genes involved in inflammation were mainly CCR8,CCL19,CCL4,IL-17,IRF1,IFN-γ,SLC11A1,etc.,involving acute inflammation,chemokine receptor activity,acute phase reaction and inflammation biological processes such as the positive regulation of reactions and inflammation.KEGG database analysis showed that differentially expressed inflammation-related genes were mainly enriched in Toll-like receptor signaling pathway,NF-κB signaling pathway,IL-17 signaling pathway,TNF signaling pathway and inflammatory bowel disease.Real-time quantitative PCR results showed IFN-γ and MALT genes were basically consistent with the transcriptome results.This experiment completed the transcriptome sequencing analysis of the duodenum of Cherry Valley duck infected by DPV,obtained the functional annotation information of differentially expressed genes,and initially revealed the inflammation-related pathways involved in DPV infection,provided a theoretical reference for further explore the pathogenic mechanism of DPV.

Key words: duck plague virus(DPV); RNA-seq; duodenum; differential expression; inflammation-related genes; regulatory pathways

CLC Number:

S855.3

TIAN Qin, ZHANG Minghua, ZHOU Bijun, CHENG Zhentao, WANG Kaigong, WEN Ming. Screening of Duodenal Mucosal Inflammation Related Genes and Regulatory Pathways in Ducks with Duck Plague Virus Infection[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3323-3333.

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Screening of Duodenal Mucosal Inflammation Related Genes and Regulatory Pathways in Ducks with Duck Plague Virus Infection

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