基于转录组测序挖掘广西麻鸡生长发育相关基因

doi:10.16431/j.cnki.1671-7236.2022.10.022

Abstract

Abstract: 【Objective】 The purpose of this study was to screen out the key genes for growth and development of Guangxi Ma chickens through transcriptome sequencing (RNA-Seq) of leg muscles at 1- and 60-day-old.【Method】 In this study,three healthy 1- and 60-day-old hens were selected,and leg muscle tissues were collected respectively.Illumina Novaseq^TM 6000 platform was used for mRNA transcriptome sequencing.DESeq2 software was used to analyze the differentially expressed genes,and GO function and KEGG pathway enrichment analysis of the differentially expressed genes were performed.The genes related to birth growth and development were further screened,and the transcriptome sequencing results were verified by Real-time quantitative PCR.【Result】 Transcriptome sequencing reaults showed that 34 296 198-41 647 642 clean reads were obtained from 6 samples.Compared with 1-day-old leg muscle,a total of 2 304 differentially expressed genes were obtained at 60-day-old leg muscle,of which 998 genes were up-regulated and 1 306 genes were down-regulated.Go function enrichment analysis found that 572 enrichment items were obtained,including 381 biological processes,70 cell components and 121 molecular functions.The enrichment analysis of KEGG pathway found that a total of 34 signal pathways were obtained.Myocardial contraction,MAPK signal pathway,tight junction,actin cytoskeleton signal pathway and adrenergic signal transduction in cardiomyocytes were related to growth and development.Through GO function and KEGG pathway enrichment analysis,5 growth and development related genes were obtained,which were myosin heavy chain 10 (MYH10),myosin heavy chain 15 (MYH15),fibroblast growth factor 10 (FGF10),fibroblast growth factor 16 (FGF16) and myostatin (GDF8) genes, respectively.MYH10,MYH15 and FGF10 were up-regulated differentially expressed genes,FGF16 and GDF8 were down-regulated differentially expressed genes.The results of Real-time quantitative PCR of the selected differentially expressed genes were consistent with the gene expression levels of transcriptome sequencing,indicating that the transcriptome sequencing results were reliable.【Conclusion】 By analyzing the transcriptome data of 1- and 60 day-old Guangxi Ma chickens,5 genes related to growth and development were obtained,including MYH10,MYH15,FGF10,FGF16 and GDF8,which provided a reference for further exploring the regulation mechanism of growth and development of Guangxi Ma chickens.

Key words: Guangxi Ma chickens; leg muscle; RNA-Seq; differentially expressed genes

CLC Number:

S831.2

CHEN Ting, LU Shengsheng, YANG Lili, LI Tianbao, ZHANG Min, JIANG Mingsheng. Based on Transcriptome Sequencing to Explore the Relationship Between Growth and Development Genes of Guangxi Ma Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3901-3908.

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Based on Transcriptome Sequencing to Explore the Relationship Between Growth and Development Genes of Guangxi Ma Chickens

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