基于RNA-Seq数据分析环格列酮和胰岛素诱导延边黄牛前脂肪细胞分化的差异表达基因

doi:10.16431/j.cnki.1671-7236.2021.07.010

Abstract

Abstract: The purpose of this study was to investigate the effects of peroxisome proliferators activated receptors (PPARγ) agonist (ciglitazone) and insulin on lipid metabolism and its regulatory mechanism in preadipocytes of Yanbian Yellow cattle.The experiments were divided into control group (CON,5% FBS),insulin treatment group (I,5% FBS+10 mg/L insulin),cycloglitazone treatment group (C,5% FBS+10 mg/L insulin+10 mg/L cycloglitazone) and insulin+cycloglitazone treatment group (IC,5% FBS+10 mg/L insulin+10 mg/L cycloglitazone).Each group was treated for 144 h,and transcriptome sequencing was performed on the preadipocytes by RNA-Seq technology,and the differentially expressed genes were analyzed by GO functional annotation and KEGG enrichment.Analysis results of differentially expressed genes(DEGs) in each treatment group showed that:Compared with control group,the number of DEGs in IC group was 1 764,276 and 569 more than that in I and C groups,respectively.There were 371 DEGs in each treatment group.The number of up-regulated genes in IC group was 974,140 and 249 more than that in I and C groups,respectively,while the number of down-regulated genes in IC group was 790,I and C groups was 654 and 470,respectively.The GO analysis of the DEGs showed that the DEGs in each treatment group were involved in the regulation of cellular processes,metabolic processes and biological processes.In terms of molecular function,it mainly included molecular function regulator,transport activity and transcriptional regulation activity.In terms of cell composition,most of the DEGs were enriched into organelles,membranes and extracellular regions.KEGG pathway analysis showed that the DEGs were mainly concentrated in FoxO signaling pathway,PPAR signaling pathway,TGF signaling pathway,p53 signaling pathway,etc.In conclusion,the regulation mechanism of preadipocyte differentiation of Yanbian Yellow cattle treated with insulin and cycloglazidone alone was different from that treated with cycloglazidone together,and the promotion effect of the two treatments on differentiation was more obvious.The results of this study provided a basis for studying the function and molecular mechanism of cycloglitazone and insulin in regulating fat production.

Key words: RNA-Seq; insulin; ciglirtazone; Yanbian Yellow cattle; preadipocytes

CLC Number:

S823

GUO Panpan, JIN Xin, SUN Jianfu, LI Xiangzi, YIN Yunhou, YAN Changguo. Analysis of Differentially Expressed Genes in Differentiation of Yanbian Yellow Cattle Preadipocytes Induced by Cycloglitazone and Insulin Based on RNA-Seq Data[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2358-2368.

References

[1] ALBRECHT E,TEUSCHER F,ENDER K,et al.Growth-and breed-related changes of marbling characteristics in cattle[J].Journal of Animal Science,2006,84(5):1067-1075.
[2] HIDEYUKI M.Identification and utilization of genes associated with beef qualities[J].Animal Science Journal,2011,82(1):1-7.
[3] CAMP H S,LI O,WISE S C.et al.Differential activaion of peroxisome proliferator-activated receptorgamma by trolitazone and rosiglitazone[J].Diabetes,2000,49(4):539-547.
[4] 王毅飞,伊娜,吴琳英,等.吡格列酮对早期脂肪细胞分化中PPARγ2启动子转录活性的影响[J].广东医学,2016,37(19):2860-2862. WANG Y F,YI N,WU L Y,et al.Effect of pioglitazone on PPARγ2 promoter transcription in early adipocyte differentiation[J].Guangdong Medicine,2016,37(19):2860-2862.(in Chinese)
[5] 陈东.基于转录组学进行肉牛不同大理石花纹等级分子机制的研究[D].北京:中国农业大学,2015. CHEN D.Transcriptome study on the molecular mechanism of the development of marbling phenotype in beef cattle[D].Beijing:China Agricultural University,2015.(in Chinese)
[6] LEE H J,JANG M,KIM H,et al.Comparative transcriptome analysis of adipose tissues reveals that ECM-receptor interaction is involved in the depot-specific adipogenesis in cattle[J].PLoS One,2013,8(6):e66267.
[7] SHENG X H,NI H M,LIU Y H.et al.RNA-Seq analysis of bovine intramuscular,subcutaneous and perirenal adipose tissues[J].Molecular Biology Reports,2014,41(3):1631-1637.
[8] WÆRP H K L,WATERS S M,MCCABE M S,et al.RNA-Seq analysis of bovine adipose tissue in heifers fed diets differing in energy and protein content[J].PLoS One,2018,13(9):e0201284.
[9] FONSECA L F S,DOS S S,GIMENEZ D F J,et al.Gene expression profiling and identification of hub genes in Nellore cattle with different marbling score levels[J].Genomies,2019,112(1):19:873-879.
[10] LI N,ZHANG Y,LI H P,et al.Differential expression of mRNA-miRNAs related to intramuscular fat content in the longissimus dorsi in Xinjiang Brown cattle[J].PLoS One,2018,13(11):e0206757.
[11] 曹功明.葡萄糖和肾上腺素对大鼠前体脂肪细胞增殖分化及转录因子表达的影响[D].兰州:西北民族大学,2010. CAO G M.The effects of glucose and epinephrine on proliferation differentiation and transcription factorexpression of rat preadipocytes[D].Lanzhou:Northwest University for Nationalities,2010.(in Chinese)
[12] SATO K,NAKANISHI N.MITSUMOTO M.Culture condition supporting adipose conversion of stromal-vaseular cells from bovine intramuscullar adipoeyte tissues[J].Journal of Veterinary Medical Science,1996,58(11):1073-1078.
[13] GUO L,LI X,TANG Q.Transcriptional regulation of adipocyte differentiation:A central role for CCAAT/enhancer-binding protein (C/EBP)β[J].Journal of Biological Chemistry,2015,290(2):755-761.
[14] FARMER S R.Transcriptional control of adipocyte formation[J].Cell Metabolism,2006,4(4):263-273.
[15] ZHONG Q,LIN C Y.CLARBE K J et al.Endothelin-1inhibits resistin secretion in 3T3-L1 adipocytes[J].Biochemical and Biophysical Research Communications,2002,296(2):383-387.
[16] 闫研,张军芳,孙斌,等.添加环格列酮对延边黄牛骨骼肌卫星细胞成脂转分化的影响[J].中国畜牧兽医,2020,47(3):714-721. YAN Y,ZHANG J F,SUN B,et al.Effect of ciglitazone on adipogenic transdifferentiation of skeletal muscle satellite cells in Yanbian Yellow cattle[J].China Animal Husbandry & Veterinary Medicine,2020,47(3):714-721.(in Chinese)
[17] 金鑫,闫研,李香子,等.胰岛素和油酸对延边黄牛前脂肪细胞分化的影响[J].中国兽医学报,2020,280(4):754-760. JIN X,YAN Y,LI X Z,et al.Effects of insulin and oleic acid on differentiation of proadipocytes of Yanbian Yellow cattle[J].Chinese Journal of Veterinary Science,2020,280(4):754-760.(in Chinese)
[18] MURUGANANDAN S,ROMAN A A,SINAL C J.Adipocyte differentiation of bone marrow-derived mesenchymal stem cells:Cross talk with the osteoblastogenic program[J].Cellular & Molecular Life Sciences,2009,66(2):236-253.
[19] DENNLER S,GOUMANS M J,TEN D P.Transforming growth factor beta signal transduction[J].Journal of Leukocyte Biology,2002,71(5):731-740.
[20] JI F,FU S J,SHEN S L,et al.The prognostic value of combined TGF-β1 and ELF in hepatocellular carcinoma[J].BMC Cancer,2015,15(1):116-127.
[21] HATA A,CHEN Y G.TGF-β signaling from receptors to Smads[J].Cold Spring Harbor Perspectives Biology,2016,8(9):a022061.
[22] HU C T,CHANG T Y,CHENG C C,et al.Snail associates with EGR-1 and SP-1 to upregulate transcriptional activation of p15INK4b[J].FEBS Journal,2010,277(5):1202-1218.
[23] MAKOWSKI L,BOORD J B,MAEDA K,et al.Lack of macrophage fatty-acid-binding protein aP2 protects mice deficient in apolipoprotein E against athero-sclerosis[J].Nature Medicine,2001,7(6):699-705.
[24] KRUSINOVA E,PELIKANAVA T.Fatty acid binding proteins in adipose tissue:A promising link between metabolic syndrome and atherosclerosis?[J].Diabetes Research & Clinical Practice,2008,82(supp-S2):127-134.
[25] 秦立红,刘伟英,曹阳,等.不同发育阶段草原红牛背最长肌FABP4基因表达水平研究[J].中国畜牧杂志,2011,47(1):21-24. QIN L H,LIU W Y,CAO Y,et al.Expression of FABP4 gene in longissimus dorsi muscle of Steppe Red cattle at different developmental stages[J].Chinese Journal of Animal Science,2011,47(1):21-24.(in Chinese)
[26] 魏胜娟.牛FABP4基因代谢功能研究及品种间脂肪细胞发育特性研究[D].杨凌:西北农林科技大学,2014. WEI S J.Metabolic function research on bovine FABP4 gene and analysis of varietal features on adipocyte development[D].Yangling:Northwest A&F University,2014(in Chinese)
[27] MAKOWSKI L,HOTAMISLIGIL G S.The role of fatty acid binding proteins in metabolic syndrome and atherosclerosis[J].Current Opinion in Lipidology,2005,16(5):543-548.
[28] FERRERO G O,VELAZQUEZ F N,CAPUTTO B L.The kinase c-Src and the phosphatase TC45 coordinately regulate c-Fos tyrosine phosphorylation and c-Fos phospholipid synthesis activation capacity[J].Oncogene,2012,31(28):3381-3391.

Analysis of Differentially Expressed Genes in Differentiation of Yanbian Yellow Cattle Preadipocytes Induced by Cycloglitazone and Insulin Based on RNA-Seq Data

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	MENG Chaoqun, LI Chengping, ZHAO Wei, QIN Xuyong, ZHOU Guoli. Effect of miR-137-3p on Adipogenic Differentiation of Preadipocytes 3T3-L1 by Targeting MAX Gene [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1928-1937.
[2]	SUN Yanyong, MA Fengying, GUO Lili, LIU Zaixia, LIU Bin, ZHANG Wenguang, LIU Yongbin. Advances on the Application of Animal Blood Transcriptome [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 460-468.
[3]	LI Mingbo, GAO Qingshan, LI Guanhao, ZHANG Kui, CUI Lianhua, HOU Lina. Effects of Chinese Herbal Medicine Feed Additives on Meat Quality and Flavor Substances in Yanbian Yellow Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 531-542.
[4]	NIE Jingru, MA Li, YAN Dawei, DENG Jun, ZHANG Hao, ZHANG Bo, LIU Jinqiao, DONG Xinxing. Analysis of Differentially Expressed Genes and Regulation Pathways of Intramuscular Fat Deposition in Large Diqing Tibetan Pigs at Different Growth Stages [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2855-2868.
[5]	ZHANG Xueping, SHI Bingang, JIN Xiayang, WANG Xiangyan, LAN Lijuan, SHI Yu, QI Youpeng, ZHAO Shijie, LI Shaobin, HU Jiang. Expression Characterization Analysis of KLF7 Gene and Effects of Its Overexpression on Proliferation and Differentiation of Preadipocytes in Tibetan Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2920-2930.
[6]	YANG Shuai, SHI Mingyue, LI Wenxia, YUN Jiale, SUN Di, LU Chang, YANG Yang, CAI Chunbo, GAO Pengfei, GUO Xiaohong, LI Bugao, CAO Guoqing. Screening and Expression Analysis of Genes Related to Porcine Intramuscular Fat Deposition [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1651-1661.
[7]	TIAN Wenjie, WANG Dandan, WANG Shengnan, MA Yuehui, JIANG Lin, SONG Ziyi. Transcriptome Analysis of Porcine Kidney with ZBED6 Gene Deletion [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1662-1670.
[8]	SU Xiaohui, HE Haiying, TUERXUNAYI·Yalikun, WANG Qiong, MA Haiyu, LIU Lingling, LIU Wujun. Identification and Analysis of Differentially Expressed lncRNA of Tail Fat in the Second Generation of Crossbred from Wild Argali×Bashibai Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1796-1805.
[9]	SUN Qingyun, FAN Xiang, XING Ya, ZHAO Minmeng, LIU Long, GENG Tuoyu, GONG Daoqing. Study on the Relationship Between OTUD7A Gene and Goose Fatty Liver Formation [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1295-1303.
[10]	WEI Yunfang, LI Feixiang, Xing Yun, Huang Qingguo, LI Judong, WAN Jiusheng, CHEN Fangliang. Study on Cloning, Bioinformatics Analysis and Spatio-temporal Expression of IGF2 Gene in Kunming Dogs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 866-875.
[11]	ZHANG Peiying, SONG Pengyan, ZHOU Ying, CHEN Xiaoyong, ZHOU Rongyan. Analysis of Differentially Expressed Genes in Glucose-induced Senescence of Sheep Follicular Granulosa Cells Based on RNA-Seq Data [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 631-640.
[12]	CUI Baoshan, HUANG Fei, WANG Jie, LI Nan, GAO Qinghua. Screening of Sex-specific Genes in vivo-Produced Bovine Blastocysts Based on Single-cell RNA Sequencing Technology [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4573-4581.
[13]	HE Xue, GU Ying, SIDENG Danba, DE Dema, DOU Xiaoning, WU Yi, LEI Zhihui, PAN Heping, YUE Yaojing. Induction of Secondary Hair Follicle Morphogenesis in Ordos Fine Wool Sheep Based on Single-cell Transcriptome Sequencing Technology [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4296-4306.
[14]	CHEN Ting, LU Shengsheng, YANG Lili, LI Tianbao, ZHANG Min, JIANG Mingsheng. Based on Transcriptome Sequencing to Explore the Relationship Between Growth and Development Genes of Guangxi Ma Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3901-3908.
[15]	CHEN Xuan, LYU Yanqiu, HAN Yue, XU Yichao, JIN Yi. Effect of Trehalose Replacing Partial Glycerol on Frozen-thawed Sperm Quality of Yanbian Yellow Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 256-264.