猪肌内脂肪沉积相关基因的筛选及其表达特性分析

doi:10.16431/j.cnki.1671-7236.2022.05.006

Abstract

Abstract: 【Objective】 The aim of this study was to screen the genes related to intramuscular fat deposition and analyze the expression characteristics based on studying the transcriptome sequencing data of longissimus dorsi muscle in Mashen and Large White pigs.【Method】 The longissimus dorsi muscle samples of Mashen pigs and Large White pigs at the age of 180 days old were collected and sequenced by RNA-Seq technology to screen differentially expressed genes.Founctional enrichment analysis of the differentially expressed genes was conducted by GO function and KEGG pathway.GeneCards was used to query gene function online and further screen differentially expressed genes related to intramuscular fat deposition.Real-time quantitative PCR was used to analyze the expression characteristics of the differentially expressed genes in different tissues of the two varieties and intramuscular adipocyte differentiation process at the different stages.【Result】 There were 280 differentially expressed genes in longissimus dorsi muscle between Mashen and Large White pigs,of which 128 were significantly up-regulated and 152 were significantly down-regulated.GO function enrichment analysis identified 46 items,including 24 in biological processe,8 in molecular function,and 14 in cellular component.Meanwhile,KEGG pathway analysis recognized that the differentially expressed genes were involved in PPAR,MAPK and adipokine signaling pathways.Six genes,TRAF2,DUSP1,ACOT4,NR4A1,SLC27A6 and PLIN5,which related to lipid deposition,were obtained by GeneCards function search.Real-time quantitative PCR results showed that the expression of NR4A1,DUSP1 and PLIN5 genes in longissimus dorsi muscle between Mashen and Large White pigs were significantly or extremely significantly different (P<0.05 or P<0.01),while the expression of TRAF2,ACOT4 and SLC27A6 genes were not significantly different (P>0.05),but the expression trend was consistent with the sequencing results.The expression of NR4A1 and PLIN5 genes in abdominal subcutaneous adipose tissue of Mashen pigs were extremely significantly higher than those in dorsal subcutaneous adipose tissue (P<0.01),whiles the expression levels of NR4A1 and DUSP1 genes in dorsal subcutaneous adipose tissue of Large White pigs were extremely significantly higher than those in abdominal subcutaneous adipose tissue (P<0.01),and the expression of PLIN5 gene of Large White pigs was significantly higher than that in abdominal subcutaneous adipose tissue (P<0.05).In the dorsal subcutaneous adipose tissues,the expression of NR4A1,DUSP1 and PLIN5 genes in Large White pigs was extremely significantly higher than those in Mashen pigs (P<0.01).In the abdominal subcutaneous adipose tissues,the expression of NR4A1 and DUSP1 genes in Large White pigs were significantly higher than those in Mashen pigs (P<0.05),and the expression of PLIN5 gene was not significantly different (P>0.05).During the porcine intramuscular adipogenic differentiation,the expression of NR4A1 gene showed a down-regulated trend,and the expression of DUSP1 and PLIN5 genes showed an up-regulated trend.【Conclusion】 In this study,three candidate genes (NR4A1,DUSP1 and PLIN5) related to porcine intramuscular fat deposition were obtained,which could provide a reference for further exploring the regulatory mechanism of porcine intramuscular fat deposition.

Key words: Mashen pigs; Large White pigs; RNA-Seq; differentially expressed genes; intramuscular fat

CLC Number:

Q786

YANG Shuai, SHI Mingyue, LI Wenxia, YUN Jiale, SUN Di, LU Chang, YANG Yang, CAI Chunbo, GAO Pengfei, GUO Xiaohong, LI Bugao, CAO Guoqing. Screening and Expression Analysis of Genes Related to Porcine Intramuscular Fat Deposition[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1651-1661.

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Screening and Expression Analysis of Genes Related to Porcine Intramuscular Fat Deposition

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