狼山鸡高、低近交组卵巢组织转录组差异分析

doi:10.16431/j.cnki.1671-7236.2021.03.019

Abstract

Abstract: In order to explore the effect of inbreeding on reproductive effects,this study selected Langshan chickens conserved in the National Chicken Genetic Resources as materials.Combining molecular markers and pedigre information,two groups of highly and low inbred chickens were established,and the reproductive performance data were recorded for both groups.Three chickens was selected as a material in each group,and ovarian tissues were taken for RNA-seq sequencing,and the functional annotation of different genes was carried out.The results showed that a total of 1 114 differential transcripts were obtained in the highly and low inbred groups,of which 783 genes were annotated,307 were up-regulated and 476 were down-regulated.GO and KEGG analysis showed that the differential genes were mainly enriched in biological processes such as ribosome biosynthesis,inflammatory response,reproduction,growth,immune system processes,and metabolic processes.Biological pathways including in folic acid biosynthesis,oocytes maturation and metabolism were also enriched by the differential genes.Functional analysis found that the differential genes (such as GGH,CPEB1,GNMT and PIWIL) were related to reproductive function.In addition,some genes related to immunity and stress (such as APOC3,HSP70,CD38 and LGMN) were also included.The results of this study were helpful for us to understand the genes and their regulatory mechanisms related to inbreeding depression of reproductive traits in Langshan chickens,and would provide a reference for the molecular mechanism of inbreeding depression of specific traits in poultry.

Key words: Langshan chickens; inbreeding; ovary; RNA-seq sequencing; differentially genes

CLC Number:

LI Guohui, CAO Yuxia, XUE Qian, ZHANG Huiyong, YIN Jianmei, ZHU Yunfen, SHEN Haiyu, DOU Xinhong, SU Yijun, WANG Kehua, ZOU Jianmin, HAN Wei. Differential Analysis of Transcriptome in the Ovaries Between the Highly and Low Inbred Langshan Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 954-962.

References

[1] SMALLBONE W,OOSTERHOUT C V,CABLE J.The effects of inbreeding on disease susceptibility:Gyrodactylus turnbulli,infection of Guppies,Poecilia reticulata[J].Experimental Parasitology,2016,167:32-37.
[2] LEROY G.Inbreeding depression in livestock species:Review and meta-analysis[J].Animal Genetics,2015,45(5):618-628.
[3] CHARLESWORTH B,CHARLESWORTH D.The geneticbasisofinbreeding depression[J].Genetical Research,1999,74(3):329-340.
[4] ENDERS L S,NUNNEY L.Sex-specific effects of inbreeding in wild-caught Drosophila melanogaster under benign and stressful conditions[J].Journal of Evolutionary Biology,2010,23(11):2309-2323.
[5] GARCIA C,AVILA V,QUESADA H,et al.Candidate transcriptomic sources of inbreeding depression in Drosophila melanogaster[J].PLoS One,2013,8(7):e70067.
[6] GARCIA C,AVILA V,QUESADA H,et al.Gene-expression changes caused by inbreeding protect against inbreeding depression in Drosophila[J].Genetics,2013,192(1):161-172.
[7] BÉRÉNOS C,ELLIS P A,PILKINGTON J G,et al.Genomic analysis reveals depression due to both individual and maternal inbreeding in a free-living mammal population[J].Molecular Ecology,2016,25(13):3152-3168.
[8] HUISMAN J,KRUUK L E B,ELLIS P A,et al.Inbreeding depression across the lifespan in a wild mammal population[J].Proceedings of the National Academy of Sciences of the United States of America,2016,113(13):3585.
[9] KRISTENSEN N T.Genome-wide analysis on inbreeding effects on gene expression in Drosophila melanogaster[J].Genetics,2005,171(1):157-167.
[10] AYROLES J F,HUGHES K A,ROWE K C,et al.A genomewide assessment of inbreeding depression:Gene number,function,and mode of action[J].Conservation Biology,2009,23(4):920-930.
[11] FRANKE K,FISCHER K.Inbreeding interferes with the heat-shock response[J].Heredity,2015,114(1):80-84.
[12] 李俊辉,罗燕秋,钟雅诗,等.近交对马氏珠母贝生长性状、遗传多样性及矿化基因表达的影响[J].南方农业学报,2017,48(1):132-138.(in Chinese) LI J H,LUO Y Q,ZHONG Y S,et al.Effects of inbreeding on growth traits,genetic diversity and biomineraliazation gene expression of Pinctada fuctada martensii[J].Journal of Southern Agriculture,2017,48(1):132-138.
[13] 曹愉夏,韩威,李国辉,等.基于RAD-seq简化基因组测序估算狼山鸡分子亲缘系数和近交系数[J].中国家禽,2019,10(41):14-19. CAO Y X,HAN W,LI G H,et al.Estimation of genomic kinship coefficients and inbreeding coefficients based on RAD-seq simplified genome sequencing data in Langshan chicken[J].China Poultry,2019,10(41):14-19.(in Chinese)
[14] LI S,ZHI L,LIU Y,et al.Effect of in ovo feeding of folic acid on the folate metabolism,immune function and epigenetic modification of immune effector molecules of broiler[J].British Journal of Nutrition,2015,1(3):1-11.
[15] LIU Y,ZHI L,SHEN J,et al.Effect ofin ovofolic acid injection on hepatic IGF2 expression and embryo growth of broilers[J].Journal of Animal Science & Biotechnology,2016,7(1):40.
[16] HEBERT K,HOUSE J D,GUENTER W.Effect of dietary folic acid supplementation on egg folate content and the performance and folate status of two strains of laying hens[J].Poultry Science,2005,84(10):1533-1538.
[17] KIM S E,COLE P D,SOHN K J,et al.Abstract 1607:Effect of folate and GGH modulation on chemosensitivity of colon and breast cancer cells to 5-fluorouracil and methotrexate[J].Cancer Research,2010,70(8):1607-1608.
[18] 林杉.营养诱导舍饲绵羊非繁殖季节发情相关microRNA筛选及其靶基因功能验证[D].石河子:石河子大学,2015. LIN S.The microRNA and target gene identify controlled by nutrition level of estrous stall-feed sheep in non-breeding season[D].Shihezi:Shihezi University,2015.(in Chinese)
[19] NISHIMURA Y,KANO K,NAITO K.Porcine CPEB1 is involved in Cyclin B translation and meiotic resumption in porcine oocytes[J].Animal Science Journal,2010,81(4):444-452.
[20] HYON C,MANSOUR-HENDILI L,CHANTOT-BASTARAUD S,et al.Deletion of CPEB1 gene:A rare but recurrent cause of premature ovarian insufficiency[J].The Journal of Clinical Endocri-nology & Metabolism,2016,101(5):2099-2104.
[21] GOMES F M,HE N,WANG F,et al.Human-specific subcellular compartmentalization of P-element induced wimpy testis-like (PIWIL) granules during germ cell development and spermatogenesis[J].Human Reproduction,2018,33(2):258-269.
[22] 陈静.种公鸡弱(无)精症发生的生理与分子机制初步研究[D].扬州:扬州大学,2015. CHEN J.Preliminary study of physiological and moleclar mechanism of chicken asthenospermia[D].Yangzhou:Yangzhou University,2015.(in Chinese)
[23] 傅强.虾夷扇贝(Patinopecten yessoensis)近交衰退的生物学效应及遗传机理研究[D].青岛:中国海洋大学,2014. FU Q.Biological effects and genetic mechanism of inbreeding depression in Japanese scallop(Patinopecten yessoensis)[D].Qingdao:Ocean University of China,2014.(in Chinese)
[24] KRISTENSEN T N,SØRENSEN P,PEDERSEN K S,et al.Inbreeding by environmental interactions affect gene expression in Drosophila melanogaster[J].Genetics,2006,173(3):1329-1336.
[25] FAH L,YONG W,XIAO L L,et al.The silencing of ApoC3 suppresses oxidative stress and inflammatory responses in placenta cells from mice with preeclampsia via inhibition of the NF-κB signaling pathway[J].Biomedicine & Pharmacotherapy,2018,107:1377-1384.
[26] MEIJER E P,COOLEN S A J,BAST A,et al.Exercise-induced oxidative stress in older adults as measured by antipyrine oxidation[J].Metabolism Clinical and Experimental,2001,50(12):1484-1488.
[27] PERIPOLLI E,METZGER J,DE LEMOS M V A,et al.Autozygosity islands and ROH patterns in Nellore lineages:Evidence of selection for functionally important traits[J].BMC Genomics,2018,19(1):680-694.

Differential Analysis of Transcriptome in the Ovaries Between the Highly and Low Inbred Langshan Chickens

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