基于RAD-seq测序的狼山鸡基因组选择信号分析

doi:10.16431/j.cnki.1671-7236.2020.09.002

Abstract

Abstract: To identify characteristic genes of Langshan chickens through genome selection signal testing,RAD-seq simplified genome sequencing technology was employed to identify SNP markers in Langshan and other 18 indigenous chicken breeds.The genetic structure and selected genes were analyzed by constructing phylogenetic tree and testing genome selective signals.The results showed that 320 874 SNPs were identified in Langshan chickens.In general,19 indigenous chicken breeds were grouped into five clusters,which were consistent with the breeding history and geographical distribution.In total,46 selected areas and 122 genes in Langshan chickens were identified through ZHp signal testing (ZHp<-3.5).Among these areas,some were also selected in WH(15 areas),BJ(14 areas),DG(11 areas) and BY((13 areas)) chickens.GO and KEGG function analysis showed that the 122 selected genes in Langshan chickens were mainly enriched in hemopoiesis,regulation of transcription,eosinophil chemotaxis and ossification processes,and adrenergic signaling in cardiomyocytes,Toll-like receptor signaling pathway,calcium signaling pathway,cytosolic DNA-sensing pathway and NOD-like receptor signaling pathway,respectively (P<0.05).The selection mainly functioned on shaping characters of stimulus-response,innate immunity,metabolism as well as nervous system development in Langshan chickens.The results could provide important genetic information for evaluation,conservation and utilization of Langshan chickens.

Key words: Langshan chickens; simplified genome sequencing; selection signal; selection genes; function analysis

CLC Number:

S813.3

ZHU Yunfen, YIN Jianmei, ZHANG Jifa, LI Guohui, SHEN Haiyu, XUE Qian, ZHANG Huiyong, DOU Xinhong, SU Yijun, HAN Wei. Selective Signal Analysis of Langshan Chickens Based on RAD-seq[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2706-2714.

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Selective Signal Analysis of Langshan Chickens Based on RAD-seq

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